Biochemical enzyme characterisations in the Fellner laboratory

The Fellner group had a productive year characterising a wide range of enzymes with this presentation focusing on the published work over the last 12 months as well as giving sneak peeks into current projects driven by group members.

We report on new chemical tools in our arsenal to target a single serine residue in the proteome of bacteria as well as deepening our general understanding of serine proteases and hydrolases. Tied to this we developed a Mass Spectrometry/Nuclear Magnetic Resonance method to study bacterial lipidomics. Other enzyme characterisations could aid in replacing fossil fuels, in improving wound healing or in combating cancer. Unpublished work expands our knowledge of protease and hydrolases in multiple ways and aims to understand bacterial stress responses better.

The presentation will also give an insight into new enzyme characterisation synchrotron techniques that are used in these studies.