Dr Louise Paton, Research, Christchurch Heart Institute

Scientific Officer (Proteomics)

PhD(Cant)

Research interests

Dr Louise Paton graduated from the University of Canterbury with a double major BSc in Biochemistry and Plant and Microbial Sciences. She completed her MSc in Plant Biotechnology before becoming employed as a protein chemistry technician at the Wool Research Organisation of New Zealand. She gained a Bright Future Enterprise Scholarship and completed her PhD under the supervision of Professor Juliet Gerrard in Biochemistry, specialising in proteomics and protein assembly. She worked as a Scientist at Canesis Inc before joining the Centre for Free Radical Research in 2005 as the Proteomics Research Manager. Louise transferred to the Christchurch Heart Institute in 2020.

Dr Paton is experienced in many biochemical and protein chemistry techniques including ELISA, chromatography, cell and bacterial culture. She has many years of experience in mass spectrometry, using proteins, peptides and small molecules, including both qualitative and quantitative methods and structural determination.

Publications

Ocariza, M. G. C., Paton, L. N., Tang, W. H. W., Templeton, E. M., Pilbrow, A. P., Pemberton, C. J., & Appleby, S. (2024). CNDP2 is a potential modulator of heart failure outcomes. Proceedings of the 17th GeneMappers Conference. Retrieved from https://www.genemappersconference.org Conference Contribution - Published proceedings: Abstract

Ocariza, M., Paton, L., Tang, W., Templeton, E., Pilbrow, A., Pemberton, C., & Appleby, S. (2024). Investigating the role of carnosine dipeptidase II (CNDP2) in heart disease. Heart, Lung & Circulation, 33(Suppl. 2), (pp. S62). doi: 10.1016/j.hlc.2024.04.228 Conference Contribution - Published proceedings: Abstract

Appleby, S., Aitken-Buck, H. M., Holdaway, M. S., Byers, M. S., Frampton, C. M., Paton, L. N., Richards, A. M., Lamberts, R. R., & Pemberton, C. J. (2024). Cardiac effects of myoregulin in ischemia-reperfusion. Peptides, 174, 171156. doi: 10.1016/j.peptides.2024.171156 Journal - Research Article

Paumann-Page, M., Ashby, L. V., Khalilova, I., Magon, N. J., Hofbauer, S., Paton, L. N., … Kettle, A. J. (2023). Hypochlorous acid inactivates myeloperoxidase inside phagocytosing neutrophils. Redox Biochemistry & Chemistry, 5-6, 100008. doi: 10.1016/j.rbc.2023.100008 Journal - Research Article

Edwards, T. S., Dickerhof, N., Magon, N. J., Paton, L. N., Sly, P. D., & Kettle, A. J. (2022). Formation of calprotectin-derived peptides in the airways of children with cystic fibrosis. Journal of Immunology, 208, 979-990. doi: 10.4049/jimmunol.2001017 Journal - Research Article

2024

Journal - Research Article

Conference Contribution - Published proceedings: Abstract

2023

Journal - Research Article

2022

Journal - Research Article

2021

Journal - Research Article

Paumann-Page, M., Kienzl, N. F., Motwani, J., Bathish, B., Paton, L. N., Magon, N. J., … Eccles, M. R., & Winterbourn, C. C. (2021). Peroxidasin protein expression and enzymatic activity in metastatic melanoma cell lines are associated with invasive potential. Redox Biology, 46, 102090. doi: 10.1016/j.redox.2021.102090

2020

Journal - Research Article

Bathish, B., Paumann-Page, M., Paton, L. N., Kettle, A. J., & Winterbourn, C. C. (2020). Peroxidasin mediates bromination of tyrosine residues in the extracellular matrix. Journal of Biological Chemistry, 295(36), 12697-12705. doi: 10.1074/jbc.RA120.014504

Dickerhof, N., Paton, L., & Kettle, A. J. (2020). Oxidation of bacillithiol by myeloperoxidase-derived oxidants. Free Radical Biology & Medicine, 158, 74-83. doi: 10.1016/j.freeradbiomed.2020.06.009

2016

Journal - Research Article

Peskin, A. V., Pace, P. E., Behring, J. B., Paton, L. N., Soethoudt, M., Bachschmid, M. M., & Winterbourn, C. C. (2016). Glutathionylation of the active site cysteines of peroxiredoxin 2 and recycling by glutaredoxin. Journal of Biological Chemistry, 291(6), 3053-3062. doi: 10.1074/jbc.M115.692798

2015

Conference Contribution - Published proceedings: Abstract

Peskin, A., Pace, P., Behring, J., Paton, L., Soethoudt, M., Bachschmid, M., & Winterbourn, C. (2015). Glutathionylation at the Active Sites of Peroxiredoxin 2 and Recycling by Glutaredoxin. Free Radical Biology & Medicine, 87(Suppl. 1), (pp. 105). doi: 10.1016/j.freeradbiomed.2015.10.276

2014

Journal - Research Article

Soethoudt, M., Peskin, A. V., Dickerhof, N., Paton, L. N., Pace, P. E., & Winterbourn, C. C. (2014). Interaction of adenanthin with glutathione and thiol enzymes: Selectivity for thioredoxin reductase and inhibition of peroxiredoxin recycling. Free Radical Biology & Medicine, 77, 331-339. doi: 10.1016/j.freeradbiomed.2014.09.025

2013

Journal - Research Article

Paumann-Page, M., Furtmüller, P. G., Hofbauer, S., Paton, L. N., Obinger, C., & Kettle, A. J. (2013). Inactivation of human myeloperoxidase by hydrogen peroxide. Archives of Biochemistry & Biophysics, 539(1), 51-62. doi: 10.1016/j.abb.2013.09.004

Peskin, A. V., Dickerhof, N., Poynton, R. A., Paton, L. N., Pace, P. E., Hampton, M. B., & Winterbourn, C. C. (2013). Hyperoxidation of peroxiredoxins 2 and 3: Rate constants for the reactions of the sulfenic acid of the peroxidatic cysteine. Journal of Biological Chemistry, 288, 14170-14177. doi: 10.1074/jbc.M113.460881

Conference Contribution - Poster Presentation (not in published proceedings)

Peskin, A. V., Dickerhof, N., Paton, L. N., Poynton, R. A., Pace, P., Hampton, M. B., & Winterbourn, C. C. (2013, August). Hyperoxidation of human peroxiredoxin 2 and 3. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

2012

Conference Contribution - Published proceedings: Abstract

Poynton, R., Peskin, A., Dickerhof, N., Paton, L., Winterbourn, C., & Hampton, M. (2012). Kinetics of peroxiredoxin 2 and 3 hyperoxidation. Proceedings of the Oxygen Theme Meeting. (pp. 14). Dunedin, New Zealand: University of Otago. Retrieved from https://www.otago.ac.nz/research/otago051864.pdf

2011

Journal - Research Article

Tidén, A.-K., Sjögren, T., Svensson, M., Bernlind, A., Senthilmohan, R., Auchère, F., … Forbes, L. V., Magon, N. J., Paton, L. N., Jameson, G. N. L., … Kettle, A. J. (2011). 2-Thioxanthines are mechanism-based inactivators of myeloperoxidase that block oxidative stress during inflammation. Journal of Biological Chemistry, 286(43), 37578-37589. doi: 10.1074/jbc.M111.266981

2010

Journal - Research Article

Paton, L. N., Mocatta, T. J., Richards, A. M., & Winterbourn, C. C. (2010). Increased thrombin-induced polymerization of fibrinogen associated with high protein carbonyl levels in plasma from patients post myocardial infarction. Free Radical Biology & Medicine, 48(2), 223-229. doi: 10.1016/j.freeradbiomed.2009.10.044

2009

Journal - Research Article

Cox, A. G., Peskin, A. V., Paton, L. N., Winterbourn, C. C., & Hampton, M. B. (2009). Redox potential and peroxide reactivity of human peroxiredoxin 3. Biochemistry, 48(27), 6495-6501. doi: 10.1021/bi900558g

2008

Journal - Research Article

Paton, L. N., Gerrard, J. A., & Bryson, W. G. (2008). Two-dimensional gel electrophoresis of wool intermediate filament proteins. Journal of Proteomics, 71, 439-447. doi: 10.1016/j.jprot.2008.06.008

Paton, L. N., Gerrard, J. A., & Bryson, W. G. (2008). Investigations into charge heterogeneity of wool intermediate filaments proteins. Journal of Proteomics, 71, 513-529. doi: 10.1016/j.jprot.2008.08.001

2007

Journal - Research Article

Plowman, J. E., Paton, L. N., & Bryson, W. G. (2007). The differential expression of proteins in the cortical cells of wool and hair fibres. Experimental Dermatology, 16, 707-714. doi: 10.1111/j.1600-0625.2007.00576.x

Peskin, A. V., Low, F. M., Paton, L. N., Maghzal, G. J., Hampton, M. B., & Winterbourn, C. C. (2007). The high reactivity of peroxiredoxin 2 with H₂O₂ is not reflected in its reaction with other oxidants and thiol reagents. Journal of Biological Chemistry, 282(16), 11885-11892.

2003

Journal - Research Article

Plowman, J. E., Flanagan, L. M., Paton, L. N., Fitzgerald, A. C., Joyce, N. I., & Bryson, W. G. (2003). The effect of oxidation or alkylation on the separation of wool keratin proteins by two-dimensional gel electrophoresis. Proteomics, 3, 942-950. doi: 10.1002/pmic.200300419