Adjunct Research Associate Professor Khoon Lim

2024

Journal - Research Article

Soliman, B. G., Chin, I. L., Li, Y., Ishii, M., Ho, M. H., Khanh, D. V., … Lindberg, G. C. J., … Woodfield, T. B. F., … Lim, K. S. (2024). Droplet-based microfluidics for engineering shape-controlled hydrogels with stiffness gradient. Biofabrication. Advance online publication. doi: 10.1088/1758-5090/ad6d8e

Abdelmoneim, D., Coates, D., Porter, G., Schmidlin, P., Li, K. C., Botter, S., Lim, K., & Duncan, W. (2024). In vitro and in vivo investigation of antibacterial silver nanoparticles functionalized bone grafting substitutes. Journal of Biomedical Materials Research Part A. Advance online publication. doi: 10.1002/jbm.a.37757

Veenendaal, L., Liu, V. Y., Lacey, C., Lim, K. S., Hooper, G. J., Woodfield, T. B. F., & Lindberg, G. C. J. (2024). Donor ethnicity, sex, and age impact chondrogenic re-differentiation capacity: A multi-demographic study of human articular chondrocytes in vitro. Journal of the Royal Society of New Zealand. Advance online publication. doi: 10.1080/03036758.2024.2340481

Norberg, A. E., Bakirci, E., Lim, K. S., Dalton, P. D., Woodfield, T. B. F., & Lindberg, G. C. J. (2024). Bioassembly of hemoglobin-loaded photopolymerizable spheroids alleviates hypoxia-induced cell death. Biofabrication, 16(2), 025026. doi: 10.1088/1758-5090/ad2a7d

Major, G., Ahn, M., C, W.-W., Santos, M., Wise, J., Phillips, E., … Woodfield, T., & Lim, K. S. (2024). Programming temporal stiffness cues within extracellular matrix hydrogels for modelling cancer niches. Materials Today Bio, 25, 101004. doi: 10.1016/j.mtbio.2024.101004

Karimi, F., Farbehi, N., Ziaee, F., Lau, K., Monfared, M., Kordanovski, M., … Lim, K. S., & Rnjak-Kovacina, J. (2024). Photocrosslinked silk fibroin microgel scaffolds for biomedical applications. Advanced Functional Materials, 2313354. doi: 10.1002/adfm.202313354

Lindberg, G., Norberg, A., Soliman, B., Jüngst, T., Lim, K., Hooper, G., … Woodfield, T. (2024). Fabrication of gelatin-heparin based cartilage models: Enhancing spatial complexity through refinement of stiffness properties and oxygen availability. Frontiers in Biomaterials Science, 3, 1331032. doi: 10.3389/fbiom.2024.1331032

Major, G., Simcock, J., Kumar, A., Kleffmann, T., Woodfield, T. B. F., & Lim, K. S. (2024). Comprehensive matrisome profiling of human adipose tissue for soft tissue reconstruction. Advanced Biology, 8, 2300448. doi: 10.1002/adbi.202300448

Longoni, A., Major, G. S., Jiang, S., Farrugia, B. L., Kieser, D. C., Woodfield, T. B. F., … Lim, K. S. (2024). Pristine gelatin incorporation as a strategy to enhance the biofunctionality of poly(vinyl alcohol)-based hydrogels for tissue engineering applications. Biomaterials Science, 12, 134-150. doi: 10.1039/d3bm01172k

Conference Contribution - Published proceedings: Abstract

Longoni, A., Arnold, S., Major, G. S., Jiang, A., Wise, L. M., Hooper, G. J., Kieser, D., Woodfield, T. B. F., … Lim, K. S. (2024). Gelatin and VEGF incorporation in PVA-tyramine hydrogels as a strategy to enhance vascular infiltration and treat avascular necrosis. Orthopaedic Proceedings, 106-B(Supp. 8), (pp. 15). doi: 10.1302/1358-992x.2024.8.015

Woodfield, T., Major, G., Longoni, A., Simcock, J., Hooper, G., & Lim, K. (2024). Developing next-generation injectable adipose tissue grafts for soft-tissue reconstruction. Orthopaedic Proceedings, 106-B(Supp. 8), (pp. 37). doi: 10.1302/1358-992x.2024.8.037

2023

Journal - Research Article

Soliman, B. G., Longoni, A., Major, G. S., Lindberg, G. C. J., Choi, Y. S., Zhang, Y. S., Woodfield, T. B. F., & Lim, K. S. (2023). Harnessing macromolecular chemistry to design hydrogel micro- and macro-environments [Review]. Macromolecular Bioscience, 2300457. Advance online publication. doi: 10.1002/mabi.202300457

Cianciosi, A., Stecher, S., Löffler, M., Bauer-Kreisel, P., Lim, K. S., Woodfield, T. B. F., … Jungst, T. (2023). Flexible allyl-modified gelatin photoclick resin tailored for volumetric bioprinting of matrices for soft tissue engineering. Advanced Healthcare Materials, 12(30), 2300977. doi: 10.1002/adhm.202300977

Vahala, D., Amos, S. E., Sacchi, M., Soliman, B. G., Hepburn, M. S., Mowla, A., … Lim, K. S., & Choi, Y. S. (2023). 3D volumetric mechanosensation of MCF7 breast cancer spheroids in a linear stiffness gradient GelAGE. Advanced Healthcare Materials, 12(31), 2301506. doi: 10.1002/adhm.202301506

Major, G., Longoni, A., Simcock, J., Magon, N. J., Harte, J., Bathish, B., Kemp, R., Woodfield, T., & Lim, K. S. (2023). Clinical applicability of visible light-mediated cross-linking for structural soft tissue reconstruction. Advanced Science, 10, 2300538. doi: 10.1002/advs.202300538

Bolam, S. M., Zhu, M. F., Lim, K. S., Konar, S., Oliver, M. H., Buckels, E., … Woodfield, T., … Musson, D. S. (2023). Combined growth factor hydrogel enhances rotator cuff enthesis healing in rat but not sheep model. Tissue Engineering Part A, 29, 449-459. doi: 10.1089/ten.TEA.2022.0215

Tran, H. A., Hoang, T. T., Maraldo, A., Do, T. N., Kaplan, D. L., Lim, K. S., & Rnjak-Kovacina, J. (2023). Emerging silk fibroin materials and their applications: New functionality arising from innovations in silk crosslinking. Materials Today, 65, 244-259. doi: 10.1016/j.mattod.2023.03.027

Dai, Y., Qiao, K., Li, D., Isingizwe, P., Liu, H., Liu, Y., Lim, K., Woodfield, T., … Cui, X. (2023). Plant-derived biomaterials and their potential in cardiac tissue repair. Advanced Healthcare Materials, 12(20), 2202827. doi: 10.1002/adhm.202202827

Abdelmoneim, D., Porter, G., Duncan, W., Lim, K., Easingwood, R., Woodfield, T., & Coates, D. (2023). Three-dimensional evaluation of the cytotoxicity and antibacterial properties of alpha lipoic acid-capped silver nanoparticle constructs for oral applications. Nanomaterials, 13, 705. doi: 10.3390/nano13040705

Joukhdar, H., Och, Z., Tran, H., Heu, C., Vasquez, G. M., Sultana, N., … Lim, K. S., … Rnjak-Kovacina, J. (2023). Imparting multi-scalar architectural control into silk materials using a simple multi-functional ice-templating fabrication platform. Advanced Materials Technologies, 2201642. doi: 10.1002/admt.202201642

Soliman, B. G., Longoni, A., Wang, M., Li, W., Bernal, P. N., Cianciosi, A., Lindberg, G. C. J., … Woodfield, T. B. F., … Lim, K. S. (2023). Programming delayed dissolution into sacrificial bioinks for dynamic temporal control of architecture within 3D-bioprinted constructs. Advanced Functional Materials, 33(8), 2210521. doi: 10.1002/adfm.202210521

Mekhileri, N. V., Major, G., Lim, K., Mutreja, I., Chitcholtan, K., Phillips, E., Hooper, G., & Woodfield, T. (2023). Biofabrication of modular spheroids as tumour-scale microenvironments for drug screening. Advanced Healthcare Materials, 12, 2201581. doi: 10.1002/adhm.202201581

Journal - Research Other

Ho, M. H., van Hilst, Q., Cui, X., Ramaswamy, Y., Woodfield, T., Rnjak-Kovacina, J., … Lim, K. S. (2023). From adhesion to detachment: Strategies to design tissue-adhesive hydrogels. Advanced NanoBiomed Research, 2300090. doi: 10.1002/anbr.202300090

Zou, Z., Li, H., Yu, K., Ma, K., Wang, Q., Tang, J., … Lim, K., Hooper, G., Woodfield, T., Cui, X., … Tian, K. (2023). The potential role of synovial cells in the progression and treatment of osteoarthritis. Exploration, 3(5), 20220132. doi: 10.1002/EXP.20220132

Levato, R., & Lim, K. S. (2023). Harnessing light in biofabrication. Biofabrication, 15, 020401. doi: 10.1088/1758-5090/acb50f

Lim, K. S., Zreiqat, H., & Gawlitta, D. (2023). Biofabrication for orthopedic, maxillofacial, and dental applications. Acta Biomaterialia, 156, 1-3. doi: 10.1016/j.actbio.2022.12.064

Daly, A. C., & Lim, K. S. (2023). High resolution lithography 3D bioprinting. Trends in Biotechnology, 41(3), 262-263. doi: 10.1016/j.tibtech.2022.11.007

Conference Contribution - Verbal presentation and other Conference outputs

Veenendaal, L., Lim, K. S., Hooper, G. J., Woodfield, T. B. F., & Lindberg, G. C. J. (2023, August). Bioprinting spheroid-based 3D invitro disease models: Screening patient specific tissue in healthy and osteoarthritic microenvironments. Verbal presentation at the 33rd Queenstown Molecular Biology Meeting (QMB), Queenstown, New Zealand.

Abdelmoneim, D., Porter, G., Duncan, W., Lim, K., Easingwood, R., Woodfield, T., & Coates, D. (2023, July). Three-dimensional evaluation of the cytotoxicity and antibacterial properties of alpha lipoic acid-capped silver nanoparticle constructs for oral applications. Verbal presentation at the Faculty of Dentistry Clinical and Research Excellence Symposium, Dunedin, New Zealand.

Lim, K. (2023, April). Spatio-temporal modulation of physical cues using a combination of macromolecular chemistry and biofabrication approaches. Verbal presentation at the 3rd Advanced Materials and 3D Printing in Medicine Workshop, Christchurch, New Zealand.

Other - Edited Journal

Zreiqat, H., Lim, K., & Gawlitta, D. (Eds.). (2023). Acta Biomaterialia, 156 [Special issue: Biofabrication for orthopedic, maxillofacial, and dental applications]. [Guest Editors].

2022

Journal - Research Article

Veenendaal, L., Longoni, A., Hooper, G. J., Lim, K. S., Woodfield, T. B. F., & Lindberg, G. C. J. (2022). 3D-bioassembly of VH-spheroids for cartilage regeneration: In vitro evaluation of chondrogenesis, fusion and lateral integration. Advanced Materials Interfaces, 9(31), 2200882. doi: 10.1002/admi.202200882

Karimi, F., Lau, K., Kim, H. N., Och, Z., Lim, K. S., Whitelock, J., … Rnjak-Kovacina, J. (2022). Surface biofunctionalization of silk biomaterials using dityrosine cross-linking. ACS Applied Materials & Interfaces, 14, 31551-31566. doi: 10.1021/acsami.2c03345

Man, K., Alcala, C., Mekhileri, N. V., Lim, K. S., Jiang, L.-H., Woodfield, T. B. F., & Yang, X. B. (2022). GelMA hydrogel reinforced with 3D printed PEGT/PBT scaffolds for supporting epigenetically-activated human bone marrow stromal cells for bone repair. Journal of Functional Biomaterials, 13, 41. doi: 10.3390/jfb13020041

Cui, X., Alcala-Orozco, C. R., Baer, K., Li, J., Murphy, C. A., Durham, M., Lindberg, G., Hooper, G. J., Lim, K. S., & Woodfield, T. B. F. (2022). 3D bioassembly of cell-instructive chondrogenic and osteogenic hydrogel microspheres containing allogeneic stem cells for hybrid biofabrication of osteochondral constructs. Biofabrication, 14(3), 034101. doi: 10.1088/1758-5090/ac61a3

Li, J., Cui, X., Lindberg, G. C. J., Alcala-Orozco, C. R., Hooper, G. J., Lim, K. S., & Woodfield, T. B. F. (2022). Hybrid fabrication of photo-clickable vascular hydrogels with additive manufactured titanium implants for enhanced osseointegration and vascularized bone formation. Biofabrication, 14(3), 034103. doi: 10.1088/1758-5090/ac6051

Qiao, K., Xu, L., Tang, J., Wang, Q., Lim, K. S., Hooper, G., Woodfield, T. B. F., … Cui, X. (2022). The advances in nanomedicine for bone and cartilage repair. Journal of Nanobiotechnology, 20, 141. doi: 10.1186/s12951-022-01342-8

Zhu, M., Tay, M. L., Lim, K. S., Bolam, S. M., Tuari, D., Callon, K., … Woodfield, T. B. F., … Musson, D. S. (2022). Novel growth factor combination for improving rotator cuff repair: A rat in vivo study. American Journal of Sports Medicine, 50(4), 1044-1053. doi: 10.1177/03635465211072557

Soliman, B. G., Major, G. S., Atienza-Roca, P., Murphy, C. A., Longoni, A., Alcala-Orozco, C. R., … Woodfield, T. B. F., & Lim, K. S. (2022). Development and characterization of gelatin-norbornene bioink to understand the interplay between physical architecture and micro-capillary formation in biofabricated vascularized constructs. Advanced Healthcare Materials, 11(2), 2101873. doi: 10.1002/adhm.202101873

Lu, Y., Cui, X., Zhang, L., Wang, X., Xu, Y., Qin, Z., … Lim, K. S., Charles, C. J., … Tang, J. (2022). The functional role of lipoproteins in atherosclerosis: Novel directions for diagnosis and targeting therapy. Aging & Disease, 13(2), 491-520. doi: 10.14336/AD.2021.0929

Kang, B., Park, Y., Hwang, D. G., Kim, D., Yong, U., Lim, K. S., & Jang, J. (2022). Facile bioprinting process for fabricating size-controllable functional microtissues using light-activated decellularized extracellular matrix-based bioinks. Advanced Materials Technologies, 7(1), 2100947. doi: 10.1002/admt.202100947

Alcala-Orozco, C. R., Mutreja, I., Cui, X., Hooper, G. J., Lim, K. S., & Woodfield, T. B. F. (2022). Hybrid biofabrication of 3D osteoconductive constructs comprising Mg-based nanocomposites and cell-laden bioinks for bone repair. Bone, 154, 116198. doi: 10.1016/j.bone.2021.116198

Tang, J., Cui, X., Zhang, Z., Xu, Y., Guo, J., Soliman, B. G., … Lim, K. S., Woodfield, T. B. F., & Zhang, J. (2022). Injection-free delivery of MSC-derived extracellular vesicles for myocardial infarction therapeutics. Advanced Healthcare Materials, 11(5), 2100312. doi: 10.1002/adhm.202100312

Major, G. S., Simcock, J. W., Woodfield, T. B. F., & Lim, K. S. (2022). Overcoming functional challenges in autologous and engineered fat grafting trends. Trends in Biotechnology, 40(1), 77-92. doi: 10.1016/j.tibtech.2021.04.006

Journal - Research Other

Rnjak-Kovacina, J., Choi, Y. S., & Lim, K. S. (2022). Biofabrication Applications. Advanced Healthcare Materials, 11(24), 2202934. doi: 10.1002/adhm.202202934

Murphy, C. A., Lim, K. S., & Woodfield, T. B. F. (2022). Next evolution in organ-scale biofabrication: Bioresin design for rapid high-resolution vat polymerization. Advanced Materials, 34, 2107759. doi: 10.1002/adma.202107759

Conference Contribution - Published proceedings: Abstract

Abdelmoneim, D., Duncan, W., Li, K. C., Porter, G., Lim, K., & Coates, D. (2022). In vitro and in vivo investigation of antibacterial bone regenerative scaffolds. New Zealand Dental Journal, 118(4), (pp. 168). [Abstract]

Gawlitta, D., Longoni, A., Pennings, I., Lindberg, G., Woodfield, T., Lim, K., & Rosenberg, A. (2022). Vitreous humor as instructive biomaterial to support mesenchymal stem cell hypertrophy and endochondral ossification. Tissue Engineering Part A, 28(Suppl. 1), 1432. doi: 10.1089/ten.tea.2022.29025.abstracts

Braxton, T., Lim, K., Alcala-Orozco, C., Joukhdar, H., Rnjak-Kovacina, J., Woodfield, T., … Yang, X. (2022). A novel silk reinforced biphasic 3D printed PEGT/PBT scaffold for osteochondral tissue engineering. Tissue Engineering Part A, 28(Suppl. 1), 806. doi: 10.1089/ten.tea.2022.29025.abstracts

Abinzano, F., Lim, K., Núñez Bernal, P., Atienza Roca, P., Albillos Sanchez, A., Otto, I., … Woodfield, T., … Levato, R. (2022). Dual-functionalized visible-light responsive gelatin bioink as cartilage binding glue and matrix for 3D chondral regeneration. Tissue Engineering Part A, 28(Suppl. 1), 790. doi: 10.1089/ten.tea.2022.29025.abstracts

Lindberg, G., Veenendaal, L., Cui, X., Alcala, C., Durham, M., Hooper, G., Lim, K., & Woodfield, T. (2022). A 3D-bioassembly platform for probing spheroid tissue fusion and integration. Tissue Engineering Part A, 28(Suppl. 1), 703. doi: 10.1089/ten.tea.2022.29025.abstracts

Lindberg, G., Bakırcı, E., Hooper, G., Lim, K., Dalton, P., & Woodfield, T. (2022). Converging melt electrowriting with high-throughput 3D-bioassembly technologies. Tissue Engineering Part A, 28(Suppl. 1), 702. doi: 10.1089/ten.tea.2022.29025.abstracts

Joukhdar, H., Och, Z., Tran, H., Hebenton, S., Lim, K., Lord, M., & Rnjak-Kovacina, J. (2022). Development of a simple multi-functional unidirectional freezing platform to engineer aligned scaffolds for tissue engineering. Tissue Engineering Part A, 28(Suppl. 3), (pp. 714). doi: 10.1089/ten.tea.2022.29036.abstracts

Longoni, A., Major, G., Atienza-Roca, P., Wise, L., Hooper, G., Kieser, D., Woodfield, T., … Lim, K. (2022). Gelatin incorporation in VEGF-loaded PVA-Tyramine hydrogels to enhance cellular interaction and vascular infiltration. Tissue Engineering Part A, 28(Suppl. 3), (pp. 642). doi: 10.1089/ten.tea.2022.29036.abstracts

Veenendaal, L., Lim, K., Hooper, G., Woodfield, T., & Lindberg, G. (2022). Probing lateral integration of engineered cartilage spheroids in healthy and diseased microenvironments: A 3D in-vitro tissue fusion model. Tissue Engineering Part A, 28(Suppl. 3), (pp. 269). doi: 10.1089/ten.tea.2022.29036.abstracts

Major, G., Longoni, A., Simcock, J., Lim, K., & Woodfield, T. (2022). Developing next generation adipose tissue grafts for soft tissue reconstruction. Tissue Engineering Part A, 28(Suppl. 3), (pp. 187). doi: 10.1089/ten.tea.2022.29036.abstracts

Braxton, T., Lim, K., Alcala-Orozco, C., Joukhdar, H., Rnjak-Kovacina, J., Woodfield, T., … Yang, X. (2022). Investigation of the potential for osteochondrial tissue regeneration via a novel biphasic 3D printed silk reinforced scaffold. Tissue Engineering Part A, 28(Suppl. 3), (pp. 685). doi: 10.1089/ten.tea.2022.29036.abstracts

Woodfield, T., Lim, K., Lindberg, G., Cui, X., Murphy, C., & Hooper, G. (2022). Designing bioink, bioresin and multicellular spheroid fusion platforms for musculoskeletal regenerative medicine and disease modelling. Tissue Engineering Part A, 28(Suppl. 3), (pp. 323). doi: 10.1089/ten.tea.2022.29036.abstracts

Rnjak-Kovacina, J., Tran, H., Karimi, F., Joukhdar, H., Cui, X., & Lim, K. (2022). Biofabrication approaches enabled by covalent crosslinking of silk. Tissue Engineering Part A, 28(Suppl. 3), (pp. 206). doi: 10.1089/ten.tea.2022.29036.abstracts

Bolam, S., Konar, S., Zhu, M., Workman, J., Lim, K., Woodfield, T., … Musson, D. (2022). Enhancement of rotator cuff healing with a combined growth factor (IGF-1, TGF-β1 and PTH) hydrogel in a rat chronic rotator cuff tear model. Proceedings of the International Combined Orthopaedic Research Society Meeting (ICORS). 51. Retrieved from https://www.2022icors.org

Conference Contribution - Verbal presentation and other Conference outputs

Lim, K. (2022, September). [Jean Leray Award]. Invited presentation at the European Society for Biomaterials (ESB) 32nd Annual Conference, Bordeaux, France.

Lim, K. (2022, April). 3D bioprinting of vascularized constructs using versatile gelatin-based bioinks. Keynote presentation at the Australasian Society for Biomaterials and Tissue Engineering (ASBTE) 27th annual Conference, Melbourne, Australia.

2021

Journal - Research Article

Levato, R., Lim, K. S., Li, W., Usua, A. U., Peña, L. B., Wang, M., … Woodfield, T. B. F., & Malda, J. (2021). High-resolution lithographic biofabrication of hydrogels with complex microchannels from low-temperature-soluble gelatin bioresins. Materials Today Bio, 12, 100162. doi: 10.1016/j.mtbio.2021.100162

Lindberg, G. C. J., Cui, X., Durham, M., Veenendaal, L., Schon, B. S., Hooper, G. J., Lim, K. S., & Woodfield, T. B. F. (2021). Probing multicellular tissue fusion of cocultured spheroids: A 3D-bioassembly model. Advanced Science, 8(22), 2103320. doi: 10.1002/advs.202103320

Aldrich, E. D., Cui, X., Murphy, C. A., Lim, K. S., Hooper, G. J., McIlwraith, C. W., & Woodfield, T. B. F. (2021). Allogeneic mesenchymal stromal cells for cartilage regeneration: A review of in vitro evaluation, clinical experience, and translational opportunities. Stem Cells Translational Medicine, 10(11), 1500-1515. doi: 10.1002/sctm.20-0552

Man, K., Mekhileri, N., Lim, K. S., Jiang, L.-H., Woodfield, T. B. F., & Yang, X. B. (2021). MI192 induced epigenetic reprogramming enhances the therapeutic efficacy of human bone marrows stromal cells for bone regeneration. Bone, 153, 116138. doi: 10.1016/j.bone.2021.116138

Li, W., Wang, M., Mille, L. S., Robledo Lara, J. A., Huerta, V., Uribe Velázquez, T., … Murphy, C. A., … Woodfield, T. B. F., Lim, K. S., & Zhang, Y. S. (2021). A smartphone-enabled portable digital light processing 3D printer. Advanced Materials, 33(35), 2102153. doi: 10.1002/adma.202102153

Yang, K.-H., Lindberg, G., Soliman, B., Lim, K., Woodfield, T., & Narayan, R. J. (2021). Effect of photoinitiator on precursory stability and curing depth of thiol-ene clickable gelatin. Polymers, 13(11), 1877. doi: 10.3390/polym13111877

Longoni, A., Li, J., Lindberg, G. C. J., Rnjak-Kovacina, J., Wise, L. M., Hooper, G. J., Woodfield, T. B. F., Kieser, D. C., & Lim, K. S. (2021). Strategies for inclusion of growth factors into 3D printed bone grafts. Essays in Biochemistry, 65(3), 569-585. doi: 10.1042/ebc20200130

Kim, H., Kang, B., Cui, X., Lee, S.-H., Lee, K., Cho, D.-W., … Woodfield, T. B. F., Lim, K. S., & Jang, J. (2021). Light-activated decellularized extracellular matrix-based bioinks for volumetric tissue analogs at the centimeter scale. Advanced Functional Materials, 31(32), 211252. doi: 10.1002/adfm.202011252

Alcala-Orozco, C. R., Cui, X., Hooper, G. J., Lim, K. S., & Woodfield, T. B. F. (2021). Converging functionality: Strategies for 3D hybrid-construct biofabrication and the role of composite biomaterials for skeletal regeneration. Acta Biomaterialia, 132, 188-216. doi: 10.1016/j.actbio.2021.03.008

Lindberg, G. C. J., Lim, K. S., Soliman, B. G., Nguyen, A., Hooper, G. J., Narayan, R. J., & Woodfield, T. B. F. (2021). Biological function following radical photo-polymerization of biomedical polymers and surrounding tissues: Design considerations and cellular risk factors. Applied Physics Reviews, 8(1), 011301. doi: 10.1063/5.0015093

Stamp, L. K., Cameron, V. A., Woodfield, T. B. F., Walker, L., Currie, M., Templeton, E., Pilbrow, A. P., Tabakakis, K., Phillips, E., & Lim, K. S. (2021). Impact of COVID-19 on health research in New Zealand: A case study of a research-intensive campus. Journal of the Royal Society of New Zealand, 51(Suppl. 1), S75-S85. doi: 10.1080/03036758.2020.1867202

Journal - Research Other

Gentile, C., Lim, K. S., & Vozzi, G. (2021). Editorial: 3D bioprinting of vascularized tissues for in vitro and in vivo applications. Frontiers in Bioengineering & Biotechnology, 9, 754124. doi: 10.3389/fbioe.2021.754124

Conference Contribution - Published proceedings: Abstract

Machado, A. A., Stuart, G. S., Lim, K. S., & Wise, L. M. (2021). Evaluation of vascular endothelial growth factor-A₁₆₅ glycoforms in angiogenic assays. New Zealand Medical Journal, 134(1547), (pp. 144). Retrieved from https://www.nzma.org.nz/journal

Braxton, T., Lim, K., Rnjak-Kovacina, J., Alcala-Orozco, C., Woodfield, T., Jiang, L.-H., … Yang, X. (2021). Characterization of a novel silk reinforced biphasic 3D-printed scaffold for osteochondral tissue regeneration. Orthopaedic Proceedings, 103-B(Suppl. 2), (pp. 4). doi: 10.1302/1358-992x.2021.2.004

Conference Contribution - Verbal presentation and other Conference outputs

Lim, K. (2021, October). Decellularised extracellular matrices as bioinks for light-based 3d-bioprinting. Verbal presentation at the 3rd Australian Bioprinting Workshop for Tissue Engineering and Regenerative Medicine, [Online].

2020

Chapter in Book - Research

Kieser, D., Soltani, S., Wyatt, M., Lim, K., & Kieser, S. (2020). Spontaneous spinal epidural haematomas. In B. Gürer (Ed.), Vascular malformations of the central nervous system. (pp. 103-111). London, UK: IntechOpen. doi: 10.1021/ic0482537

Journal - Research Article

Baptista, M., Jouhkdar, H., Alcala-Orozco, C. R., Lau, K., Jiang, S., Cui, X., … Woodfield, T. B. F., Lim, K. S., & Rnjak-Kovacina, J. (2020). Silk fibroin photo-lyogels containing microchannels as a biomaterial platform for in situ tissue engineering. Biomaterials Science, 8, 7093-7105. doi: 10.1039/d0bm01010c

Atienza-Roca, P., Kieser, D., Cui, X., Bathish, B., Ramaswamy, Y., Hooper, G. J., Clarkson, A. N., … Wise, L. M., Woodfield, T. B. F., & Lim, K. S. (2020). Visible light mediated PVA-tyramine hydrogels for covalent incorporation and tailorable release of functional growth factors. Biomaterials Science, 8(18), 5005-5019. doi: 10.1039/D0BM00603C

Zhang, J., Cui, X., Guo, J., Cao, C., Zhang, Z., Wang, B., … Lim, K., Woodfield, T., … Zhang, J. (2020). Small but significant: Insights and new perspectives of exosomes in cardiovascular disease. Journal of Cellular & Molecular Medicine, 24, 8291-9303. doi: 10.1111/jcmm.15492

Cui, X., Li, J., Hartanto, Y., Durham, M., Tang, J., Zhang, H., Hooper, G., Lim, K., & Woodfield, T. (2020). Advances in extrusion 3D bioprinting: A focus on multicomponent hydrogel-based bioinks. Advanced Healthcare Materials, 9, 1901648. doi: 10.1002/adhm.201901648

Lim, K. S., Abinzano, F., Nuñez Bernal, P., Albillos Sanchez, A., Atienza-Roca, P., Otto, I. A., … Woodfield, T. B. F., … Levato, R. (2020). One-step photoactivation of a dual-functionalized bioink as cell carrier and cartilage-binding glue for chondral regeneration. Advanced Healthcare Materials, 9, 1901792. doi: 10.1002/adhm.201901792

Soliman, B. G., Lindberg, G. C. J., Jungst, T., Hooper, G. J., Groll, J., Woodfield, T. B. F., & Lim, K. S. (2020). Stepwise control of crosslinking in a one-pot system for bioprinting of low-density bioinks. Advanced Healthcare Materials, 9, 1901544. doi: 10.1002/adhm.201901544

Lim, K. S., Galarraga, J. H., Cui, X., Lindberg, G. C. J., Burdick, J. A., & Woodfield, T. B. F. (2020). Fundamentals and applications of photo-cross-linking in bioprinting. Chemical Reviews, 120, 10662-10694. doi: 10.1021/acs.chemrev.9b00812

Li, J., Mutreja, I., Hooper, G. J., Clinch, K., Lim, K., Evans, G., & Woodfield, T. B. F. (2020). Combined infection control and enhanced osteogenic differentiation capacity on additive manufactured Ti-6Al-4V are mediated via titania nanotube delivery of novel biofilm inhibitors. Advanced Materials Interfaces, 7, 1901963. doi: 10.1002/admi.201901963

Li, J., Cui, X., Hooper, G. J., Lim, K. S., & Woodfield, T. B. F. (2020). Rational design, bio-functionalization and biological performance of hybrid additive manufactured titanium implants for orthopaedic applications: A review. Journal of the Mechanical Behavior of Biomedical Materials, 105, 103671. doi: 10.1016/j.jmbbm.2020.103671

Cui, X., Soliman, B. G., Alcala-Orozco, C. R., Li, J., Vis, M. A. M., Santos, M., … Woodfield, T. B. F., … Lim, K. S. (2020). Rapid photocrosslinking of silk hydrogels with high cell density and enhanced shape fidelity. Advanced Healthcare Materials, 9, 1901667. doi: 10.1002/adhm.201901667

Alcala-Orozco, C. R., Mutreja, I., Cui, X., Kumar, D., Hooper, G. J., Lim, K. S., & Woodfield, T. B. F. (2020). Design and characterisation of multi-functional strontium-gelatin nanocomposite bioinks with improved print fidelity and osteogenic capacity. Bioprinting, 18, e00073. doi: 10.1016/j.bprint.2019.e00073

Conference Contribution - Published proceedings: Abstract

Lim, K. S. (2020). Engineering the vasculature for bone engineering [Invited]. Proceedings of the Australian and New Zealand Bone and Mineral Society (ANZBMS) 30th Annual Scientific Meeting. 39. Retrieved from https://www.anzbms.org.au/

Other Research Output

Lim, K. (2020, June). Harnessing macromolecular chemistry to engineer blood vessels. UOC Research Seminar Series, University of Otago, Christchurch, New Zealand. [Research Presentation].

2019

Journal - Research Article

Vanamala, R., Hammer, N., Soltani, S., Lim, K., Wyatt, M. C., & Kieser, D. C. (2019). Anatomical variations of the saphenous nerve in the adductor canal. Journal of Anesthesiology & Clinical Science, 8, 2. doi: 10.7243/2049-9752-8-2

Nugent, M., May, J. H., Parker, J. D., Kieser, D. C., Douglas, M., Pereira, R., Lim, K. S., & Hooper, G. J. (2019). Does tranexamic acid reduce knee swelling and improve early function following arthroscopic meniscectomy? A double-blind randomized controlled trial. Orthopaedic Journal of Sports Medicine, 7(8), 2325967119866122. doi: 10.1177/2325967119866122

Klotz, B. J., Oosterhoff, L. A., Utomo, L., Lim, K. S., Vallmajo-Martin, Q., Clevers, H., Woodfield, T. B. F., … Gawlitta, D. (2019). A versatile biosynthetic hydrogel platform for engineering of tissue analogues. Advanced Healthcare Materials, 8, 1900979. doi: 10.1002/adhm.201900979

Parrish, J., Lim, K., Zhang, B., Radisic, M., & Woodfield, T. B. F. (2019). New frontiers for biofabrication and bioreactor design in microphysiological system development. Trends in Biotechnology, 37(12), 1327-1343. doi: 10.1016/j.tibtech.2019.04.009

Lim, K. S., Baptista, M., Moon, S., Woodfield, T. B. F., & Rnjak-Kovacina, J. (2019). Microchannels in development, survival, and vascularisation of tissue analogues for regenerative medicine. Trends in Biotechnology, 37(11), 1189-1201. doi: 10.1016/j.tibtech.2019.04.004

Cidonio, G., Alcala-Orozco, C. R., Lim, K. S., Glinka, M., Mutreja, I., Kim, Y.-H., … Woodfield, T. B. F., & Oreffo, R. O. C. (2019). Osteogenic and angiogenic tissue formation in high fidelity nanocomposite Laponite-gelatin bioinks. Biofabrication, 11, 035027. doi: 10.1088/1758-5090/ab19fd

Lim, K. S., Klotz, B. J., Lindberg, G. C. J., Melchels, F. P. W., Hooper, G. J., Malda, J., … Woodfield, T. B. F. (2019). Visible light cross-linking of gelatin hydrogels offers an enhanced cell microenvironment with improved light penetration depth. Macromolecular Bioscience, 19(6), 1900098. doi: 10.1002/mabi.201900098

Soltani, S., Nogaro, M. C., Rougelot, C., Newell, N., Lim, K., & Kieser, D. C. (2019). Spontaneous spinal epidural haematomas in children. European Spine Journal, 28, 2229-2236. doi: 10.1007/s00586-019-05975-6

Mutreja, I., Warring, S. L., Lim, K. S., Swadi, T., Clinch, K., Mason, J. M., … Chambers, S. T., … Gerth, M. L., … Woodfield, T. B. F. (2019). Biofilm inhibition via delivery of novel methylthioadenosine nucleosidase inhibitors from PVA-tyramine hydrogels while supporting mesenchymal stromal cell viability. ACS Biomaterials Science & Engineering, 5(2), 748-758. doi: 10.1021/acsbiomaterials.8b01141

Lindberg, G. C. J., Longoni, A., Lim, K. S., Rosenberg, A. J., Hooper, G. J., Gawlitta, D., & Woodfield, T. B. F. (2019). Intact vitreous humor as a potential extracellular matrix hydrogel for cartilage tissue engineering applications. Acta Biomaterialia, 85, 117-130. doi: 10.1016/j.actbio.2018.12.022

Conference Contribution - Published proceedings: Abstract

Brewster, J., Lim, K. S., Yang, X., & Jiang, L. H. (2019). Culturing neural tissue on biocompatible graphene oxide thin films in development of tissue engineering scaffolds. Proceedings of the Tissue Engineering and Regenerative Medicine International Society European Chapter Meeting (TERMIS EU). (pp. 1066). Retrieved from https://termis.org/index.php?q=chapters-europe

Abinzano, F., Lim, K., Núñez Bernal, P., Atienza Roca, P., Albillos Sánchez, A., Otto, I. A., Woodfield, T., … Levato, R. (2019). One-step dual photocrosslinkable tyramine-enhanced bioglue for in situ tissue repair. Proceedings of the European Society for Biomaterials (ESB) 30th Annual Conference and the German Society for Biomaterials (DGBM) 26th Annual Conference. PS2-07-355. Retrieved from https://www.esb2019.org/

Tang, F., Baptista, M., Lin, X., Jiang, S., Alcala-Orozco, C., Whitelock, J., Lim, K., … Rnjak-Kovacina, J. (2019). Biometric materials toward enhanced bioengineered tissue vascularisation. Proceedings of the European Society for Biomaterials (ESB) 30th Annual Conference and the German Society for Biomaterials (DGBM) 26th Annual Conference. X-OS37-04. Retrieved from https://www.esb2019.org/

Cui, S., Li, J., Alcala-Orozco, C., Woodfield, T., Rnjak-Kovacina, J., & Lim, K. (2019). Rapid visible light crosslinking of silk hydrogels for cartilage tissue engineering. Proceedings of the European Society for Biomaterials (ESB) 30th Annual Conference and the German Society for Biomaterials (DGBM) 26th Annual Conference. X-OS19-04. Retrieved from https://www.esb2019.org/

Conference Contribution - Poster Presentation (not in published proceedings)

Aregueta-Robles, U. A., Martens, P., Lim, K. S., Poole-Warren, L., & Green, R. (2019, April). Delivering neuronal networks with degradable bio-synthetic hydrogels. Poster session presented at the Australasian Society for Biomaterials and Tissue Engineering (ASBTE) Early Career Researcher Workshop, Sydney, Australia.

Alcala-Orozco, C. R., Mutreja, I., Cui, X., Hooper, G. J., Lim, K. S., & Woodfield, T. B. F. (2019, April). Strontium-based nanocomposite bioink for 3D bioprinting of high shape-fidelity osteoconductive scaffolds. Poster session presented at the Australasian Society for Biomaterials and Tissue Engineering (ASBTE) Early Career Researcher Workshop, Sydney, Australia.

Conference Contribution - Verbal presentation and other Conference outputs

Lim, K. (2019, October). Tailoring physico-mechanical properties of bioinks for 3D extrusion bioprinting of vascularized constructs [Invited]. Verbal presentation at the Tissue Engineering & Regenerative Medicine International Society Asia Pacific Chapter (TERMIS-AP) & the 7th Asian Biomaterials Congress (ABMC7), Brisbane, Australia.

Lim, K. (2019, October). 3D bioprinting of cartilage-binding bioink for chondral regeneration [Invited]. Verbal presentation at the Tissue Engineering & Regenerative Medicine International Society Asia Pacific Chapter (TERMIS-AP) & the 7th Asian Biomaterials Congress (ABMC7), Brisbane, Australia.

Lam Po Tang, E. J., Alcala-Orozco, C. R., Nielsen, P. M. F., Taberner, A. J., Woodfield, T. B. F., & Lim, K. S. (2019, August-September). The effects of different buffers on the physico-mechanical properties of gelatin-methacryloyl (GelMA) hydrogels. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Alcala-Orozco, C. R., Cidonio, G., Lim, K. S., Dawson, J., Oreffo, R. O. C., & Woodfield, T. B. F. (2019, August-September). High fidelity bioprinting of nanocomposite bioinks supporting osteogenic and angiogenic tissue formation. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Soliman, B. G., Lindberg, G. C. J., Woodfield, T. B. F., & Lim, K. S. (2019, August-September). Two-step reuthenium-catalysed bioprinting of low-viscous cell-instructive bioinks as a platform for tissue-specific bioink development. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Woodfield, T. B. F., Lim, K. S., Lindberg, G. C. J., Cui, S., Alcala-Orozco, C., & Hooper, G. J. (2019, August-September). Biofabrication of the musculoskeletal tissue niche: Tissue spheroid and bioink platiforms. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Aldrich, E. D., Cui, S., Lim, K. S., & Woodfield, T. (2019, August-September). The horse as a model and translational stepping stone in orthopaedic research. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Atienza Roca, P., Woodfield, T., & Lim, K. (2019, August-September). Photo-click gelatin-norbornene hydrogels for biofabrication of vascularized tissue. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Zhu, M., Musson, D., Coleman, B., Lim, K., Cornish, J., & Munro, J. (2019, September-October). Evaluating a new growth factor combination for improving rotator cuff repair: A rat in vivo study. Verbal presentation at the New Zealand Orthopaedic Association (NZOA) Annual Scientific Meeting, Dunedin, New Zealand.

Hooper, G. J., May, J., Nugent, M., Douglas, M., Parker, J., & Lim, K. (2019, May). Is tranexamic acid useful in reducing knee swelling and improving early function following arthroscopic meniscectomy? Verbal presentation at the 12th Biennial International Society of Arthroscopy, Knee Surgery & Orthopaedic Sports Medicine (ISAKOS) Congress, Cancun, Mexico.

2018

Chapter in Book - Research

Atienza Roca, P., Cui, X., Hooper, G. J., Woodfield, T. B. F., Lim, K. S., & Khang, G. (2018). Growth factor delivery systems for tissue engineering and regenerative medicine. In H. J. Chun, C. H. Park & I. K. Kwon (Eds.), Advances in experimental medicine and biology: (Vol. 1078) Cutting-edge enabling technologies for regenerative medicine. (pp. 245-269). Singapore: Springer. doi: 10.1007/978-981-13-0950-2_13

Lim, K. S., Martens, P., & Poole-Warren, L. (2018). Biosynthetic hydrogels for cell encapsulation. In J. Li, Y. Osada & J. Cooper-White (Eds.), Functional hydrogels as biomaterials. (pp. 1-29). Berlin, Germany: Springer. doi: 10.1007/978-3-662-57511-6_1

Journal - Research Article

Parrish, J., Lim, K. S., Baer, K., Hooper, G. J., & Woodfield, T. B. F. (2018). A 96-well microplate bioreactor platform supporting individual dual perfusion and high-throughput assessment of simple or biofabricated 3D tissue models. Lab on a Chip, (18), 2757-2775. doi: 10.1039/c8lc00485d

Klotz, B. J., Lim, K. S., Chang, Y. X., Soliman, B. G., Pennings, I., Melchels, F. P. W., Woodfield, T. B. F., … Gawlitta, D. (2018). Engineering of a complex bone tissue model with endothelialised channels and capillary-like networks. European Cells & Materials, 35, 335-349. doi: 10.22203/eCM.v035a23

Lim, K. S., Levato, R., Costa, P. F., Castilho, M. D., Alcala-Orozco, C. R., van Dorenmalen, K. M. A., … Hooper, G. J., … Woodfield, T. B. F. (2018). Bio-resin for high resolution lithography-based biofabrication of complex cell laden constructs. Biofabrication, 10, 034101. doi: 10.1088/1758-5090/aac00c

Parker, J. D., Lim, K. S., Kieser, D. C., Woodfield, T. B. F., & Hooper, G. J. (2018). Is tranexamic acid toxic to articular cartilage when administered topically? What is the safe dose? Bone & Joint Journal, 100-B(3), 404-412. doi: 10.1302/0301-620X.100B3.BJJ-2017-1135.R1

Mekhileri, N. V., Lim, K. S., Brown, G. C. J., Mutreja, I., Schon, B. S., Hooper, G. J., & Woodfield, T. B. F. (2018). Automated 3D bioassembly of micro-tissues for biofabrication of hybrid tissue engineered constructs. Biofabrication, 10(2). doi: 10.1088/1758-5090/aa9ef1

Conference Contribution - Published proceedings: Abstract

Wise, J., Lim, K., Morrin, H., Woodfield, T., Currie, M., & Phillips, E. (2018). Engineering a 3D model system for studying breast cancer-adipocyte interactions within the tumour microenvironment. Proceedings of the New Zealand Society for Oncology (NZSO) Conference. Retrieved from http://www.nzsoncology.org.nz/

Lim, K. S., Atienza Roca, P., Anten, J., & Woodfield, T. (2018). Covalent incorporation and smart release of growth factors from photo-polymerisable PVA-Tyramine hydrogels. Proceedings of the Australasian Society for Biomaterials and Tissue Engineering (ASBTE) 26th Annual Conference. (pp. 55). Retrieved from https://www.asbte.org

Alcala Orozco, C., Mutreja, I., Lim, K., Hooper, G., & Woodfield, T. (2018). Strontium-based nanocomposite bioinks for improved osteogenic differentiation of mesenchymal stromal cells. Proceedings of the Australasian Society for Biomaterials and Tissue Engineering (ASBTE) 26th Annual Conference. (pp. 53). Retrieved from https://www.asbte.org

Lim, K., & Woodfield, T. (2018). Development of a visible light photoinitiating system for multiple biofabrication platforms. Proceedings of the Australasian Society for Biomaterials and Tissue Engineering (ASBTE) 26th Annual Conference. (pp. 19). Retrieved from https://www.asbte.org

Conference Contribution - Poster Presentation (not in published proceedings)

Brown, G., Longoni, A., Lim, K., Rosenberg, A., Hooper, G., Gawlitta, D., & Woodfield, T. (2018, September). Vitreous humor as an extracellular matrix hydrogel for cartilage tissue engineering applications. Poster session presented at the Tissue Engineering and Regenerative Medicine International Society (TERMIS) 5th World Congress, Kyoto, Japan.

Lim, K. S., Atienza Roca, P., Brown, G. C. J., Hooper, G. J., & Woodfield, T. B. F. (2018, September). Photo-click gelatin-norbornene hydrogels for tissue vascularisation. Poster session presented at the Tissue Engineering and Regenerative Medicine International Society (TERMIS) 5th World Congress, Kyoto, Japan.

Secker, D. J., Lim, K. S., Brown, G. C. J., Woodfield, T. B. F., Jiang, L.-H., Jia, X., & Yang, X. B. (2018, September). Crosslinker selection determines the properties of GelNOR hydrogels for tissue engineering applications. Poster session presented at the Tissue Engineering and Regenerative Medicine International Society (TERMIS) 5th World Congress, Kyoto, Japan.

Glinka, M., Cidonio, G., Geng, J., Czekańska, E., Kim, Y.-H., Dawson, J., … Lim, K. S., Woodfield, T., … Oreffo, R. O. C. (2018, September). Enhancing bone repair by incorporation of innovative nanoparticles containing osteogenic factors in gelatin methacryloyl hydrogel scaffolds. Poster session presented at the Tissue Engineering and Regenerative Medicine International Society (TERMIS) 5th World Congress, Kyoto, Japan.

Lim, K. S., Brown, G. C. J., Soliman, B. G., Hooper, G. J., Groll, J., & Woodfield, T. B. F. (2018, September). Photo-click thiol-ene gelatin based hydrogels as bioinks for bioprinting. Poster session presented at the Tissue Engineering and Regenerative Medicine International Society (TERMIS) 5th World Congress, Kyoto, Japan.

Cidonio, G., Alcala-Orozco, C. R., Glinka, M., Mutreja, I., Kim, Y., Dawson, J. L., Lim, K. S., Woodfield, T. B. F., & Oreffo, R. O. C. (2018, October). A nanocomposite bioink to sustain human skeletal stem cell viability and drug delivery. Poster session presented at the International Society for Biofabrication Annual Meeting, Würzburg, Germany.

Wise, J., Lim, K., Currie, M., Morrin, H., Woodfield, T., & Phillips, E. (2018, August). Development of a 3D breast cancer-adipocyte model: A tool for studying tumour microenvironment interactions in vitro. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Conference Contribution - Verbal presentation and other Conference outputs

Levato, R., Lim, K., Gawlitta, D., Woodfield, T., & Malda, J. (2018, September). Biofabrication of 3D convoluted in-hydrogel vessel-like microfluidic channels via digital light processing bioprinting. Verbal presentation at the Tissue Engineering and Regenerative Medicine International Society (TERMIS) 5th World Congress, Kyoto, Japan.

Man, K., Mekhileri, N., Schon, B., Lim, K., Jiang, L.-H., Foster, R., Woodfield, T., & Yang, X. (2018, September). Using a novel histone deacetylase inhibitor to enhance human bone marrow stromal cell osteogenic differentiation in vitro. Verbal presentation at the Tissue Engineering and Regenerative Medicine International Society (TERMIS) 5th World Congress, Kyoto, Japan.

Lim, K. (2018, October). 3D bioassembly of physiologically relevant breast cancer models. Verbal presentation at the International Society for Biofabrication Annual Meeting, Würzburg, Germany.

Alcala-Orozco, C. R., Mutreja, I., Lim, K. S., Lindberg, G., Hooper, G. J., & Woodfield, T. (2018, October). Strontium-based nanocomposite bioink for 3D-bioprinting of scaffolds with long-term shape retention and improved osteogenic differentiation of mesenchymal stromal cells. Verbal presentation at the International Society for Biofabrication Annual Meeting, Würzburg, Germany.

Lindberg, G., Lim, K., Soliman, B., Bertlein, S., Jungst, T., Hooper, G., … Woodfield, T. (2018, October). Photo-clickable allylated gelatin and thiolated heparin as chondro-instructive bioinks for 3D-biofabrication. Verbal presentation at the International Society for Biofabrication Annual Meeting, Würzburg, Germany.

Lim, K. (2018, April). Development of a new class of visible light photocatalytic hydrogels for multiple biofabrication platforms: Clinical and commercial application. Verbal presentation at the University of Otago Centre for Bioengineering & Nanomedicine Symposium: Biomaterials and Regenerative Medicine, Dunedin, New Zealand.

Lindberg, G. C. J., Lim, K. S., Soliman, B. G., Hooper, G. J., & Woodfield, T. B. F. (2018, August). Photo-curable thiol-ene gelatin-heparin hydrogels for 3D-biofabrication of functional cartilage. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Woodfield, T., Lim, K. S., Brown, G., Schon, B. S., Mekhileri, N. V., Hooper, G. J., … Groll, J. (2018, August). Designing bio-ink and bio-resin platforms for 3D bioprinting and bioassembly. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

Lim, K., Lindberg, G. C. J., & Woodfield, T. B. F. (2018, December). Design of chrondro-instructive hydrogels for high-throughput 3D-biofabrication of cartilage tissue modules. Verbal presentation at the 41st Scientific Meeting of the Matrix Biology Society of Australia and New Zealand (MBSANZ), Auckland, New Zealand.

Lim, K., & Woodfield, T. (2018, December). Development of novel visible light photo-initiators for 3D bioprinting. Verbal presentation at the 41st Scientific Meeting of the Matrix Biology Society of Australia and New Zealand (MBSANZ), Auckland, New Zealand.

Cui, X., Durham, M., Li, J., Aldrich, E., Lim, K., & Woodfield, T. (2018, December). Improving chondrogenesis of equine umbilical cord blood-mesenchymal stem cell in three-dimensional hydrogel by synergistic control of chemical and mechanical cues. Verbal presentation at the 41st Scientific Meeting of the Matrix Biology Society of Australia and New Zealand (MBSANZ), Auckland, New Zealand.

2017

Chapter in Book - Research

Woodfield, T., Lim, K., Morouço, P., Levato, R., Malda, J., & Melchels, F. (2017). Biofabrication in tissue engineering. In P. Ducheyne, D. W. Grainger, K. E. Healy, D. W. Hutmacher & C. J. Kirkpatrick (Eds.), Comprehensive biomaterials II: (Vol. 5) Tissue Engineering and regenerative medicine: Fundamentals. (2nd ed.) (pp. 236-266). Amsterdam, The Netherlands: Elsevier. doi: 10.1016/B978-0-12-803581-8.10221-8

Journal - Research Article

Bertlein, S., Brown, G., Lim, K. S., Jungst, T., Boeck, T., Blunk, T., … Hooper, G. J., Woodfield, T. B. F., & Groll, J. (2017). Thiol-ene clickable gelatin: A platform bioink for multiple 3D biofabrication technologies. Advanced Materials, 29(44), 1703404. doi: 10.1002/adma.201703404

Brown, G. C. J., Lim, K. S., Farrugia, B. L., Hooper, G. J., & Woodfield, T. B. F. (2017). Covalent incorporation of heparin improves chondrogenesis in photocurable gelatin-methacryloyl hydrogels. Macromolecular Bioscience, 17(12), 1700158. doi: 10.1002/mabi.201700158

Conference Contribution - Published proceedings: Abstract

Bertlein, S., Brown, G. C., Lim, K. S., Juengst, T., Boeck, T., Blunk, T., … Woodfield, T., & Groll, J. (2017). Allyl glycidyl ether modified gelatin as a versatile bioink platform for biofabrication of tissue engineered constructs. Proceedings of the Tissue Engineering and Regenerative Medicine International Society European Chapter Meeting (TERMIS-EU). 0195. Retrieved from http://www.ecmconferences.org

Klotz, B., Lim, K., Vallmajó Martin, Q., Pennings, I., Rosenberg, T., Woodfield, T., … Gawlitta, D. (2017). Development of a novel bio-synthetic gelatin-poly(ethylene glycol) hydrogel for pre-vascularized osteogenically differentiated tissue analogues with enhanced structural stability. Proceedings of the Tissue Engineering and Regenerative Medicine International Society European Chapter Meeting (TERMIS-EU). 0194. Retrieved from http://www.ecmconferences.org

Lim, K., & Woodfield, T. (2017). New visible light initiating system for 3D bioprinting in tissue engineering and regenerative medicine. Proceedings of the Tissue Engineering and Regenerative Medicine International Society European Chapter Meeting (TERMIS-EU). 0079. Retrieved from http://www.ecmconferences.org

Brown, G., Soliman, B., Bertlein, S., Groll, J., Lim, K., & Woodfield, T. (2017). Photo-curable thiol-ene gelatin based hydrogel bio-inks for 3D-biofabrication of cartilage. Proceedings of the Tissue Engineering and Regenerative Medicine International Society European Chapter Meeting (TERMIS-EU). 0078. Retrieved from http://www.ecmconferences.org

Phillips, E., Lim, K., Woodfield, T., & Currie, M. J. (2017). Towards 3D culture models for the investigation of adipocyte/tumour interaction. New Zealand Medical Journal, 130(1459), (pp. 79). Retrieved from http://www.nzma.org.nz/journal

Conference Contribution - Poster Presentation (not in published proceedings)

Brown, G. C. J., Lim, K., Bertlein, S., Hooper, G., Groll, J., & Woodfield, T. (2017, June-July). Covalent incorporation of heparin improves chondrogenic tissue formation for 3D bioprinting of photo-curable thiol-ene gelatin based hydrogel bioinks. Poster session presented at the International Cartilage Repair Society (ICRS) Heritage Summit, Gothenburg, Sweden.

Conference Contribution - Verbal presentation and other Conference outputs

Lim, K. (2017, October). Photo-clickable thiol-ene gelatin based biomaterial for 3D bioplotting, 3D bioassembly and digital light processing. Verbal presentation at the International Conference on Biofabrication, Beijing, China.

Lim, K. (2017, October). Development of a transition metal based visible light photoinitiator system for multiple biofabrication platforms. Verbal presentation at the International Conference on Biofabrication, Beijing, China.

Hooper, G., Parker, J., Lim, K., Kieser, D., & Woodfield, T. (2017, October). Chondro-toxicity of tranxenamic acid (TVA): A systematic evaluation using 2D and 3D cell culture models as well as human articular cartilage explants. Verbal presentation at the New Zealand Orthopaedic Association (NZOA) Annual Scientific Meeting, Auckland, New Zealand.

Atienza Roca, P., Soliman, B., Anten, J., Brown, G., Woodfield, T., & Lim, K. (2017, September). Photo-click gelatin-norbonene hydrogels for biofabrication of vascularized tissue. Verbal presentation at the Future Investigators of Regenerative Medicine (FIRM) Symposium, Girona, Spain.

Lim, K., & Woodfield, T. (2017, August). Light activated hydrogels as bioinks for 3D biofabrication. Verbal presentation at the Annual Meeting of Korean Society for Stem Cell Research, Seoul, Republic of Korea.

Atienza Roca, P., Anten, J., Brown, G., Woodfield, T., & Lim, K. (2017, April). Photo-click gelatin-norbornene hydrogels for tissue vascularisation. Verbal presentation at the Australasian Society for Biomaterials and Tissue Engineering (ASBTE) 25th Annual Conference and the 7th Indo-Australian Conference on Biomaterials, Implants, Tissue Engineering and Regenerative Medicine (BiTERM), Canberra, Australia.

Aregueta-Robles, U. A., Lim, K. S., Martens, P. J., Lovell, N. H., Poole-Warren, L. A., & Green, R. A. (2017, April). Engineering a living electrode: Growth of neural networks within a 3D hydrogel. Verbal presentation at the Australasian Society for Biomaterials and Tissue Engineering (ASBTE) 25th Annual Conference and the 7th Indo-Australian Conference on Biomaterials, Implants, Tissue Engineering and Regenerative Medicine (BiTERM), Canberra, Australia.

Lim, K., Levato, R., Costa, P., Castilho, M., van Dorenmalen, K., Melchels, F., … Woodfield, T. (2017, April). Digital light processing of a novel visible light bio-resin for 3D bioprinting of complex cell-laden constructs with high spatial resolution. Verbal presentation at the Australasian Society for Biomaterials and Tissue Engineering (ASBTE) 25th Annual Conference and the 7th Indo-Australian Conference on Biomaterials, Implants, Tissue Engineering and Regenerative Medicine (BiTERM), Canberra, Australia.

Brown, G., Bertlein, S., Lim, K., Groll, J., & Woodfield, T. (2017, April). Photo-curable thiol-ene gelatin based hydrogels as bioink. Verbal presentation at the Australasian Society for Biomaterials and Tissue Engineering (ASBTE) 25th Annual Conference and the 7th Indo-Australian Conference on Biomaterials, Implants, Tissue Engineering and Regenerative Medicine (BiTERM), Canberra, Australia.

2016

Chapter in Book - Research

Ramaswamy, Y., Lim, K. S., Zreiqat, H., & Lu, Z. (2016). Stem cells for bone regeneration: Role of trophic factors. In A. Zorzi (Ed.), Advanced techniques in bone regeneration. (pp. 357-374). Rijeka, Croatia: Intech. doi: 10.5772/63357

Journal - Research Article

Roberts, J. J., Naudiyal, P., Lim, K. S., Poole-Warren, L. A., & Martens, P. J. (2016). A comparative study of enzyme initiators for crosslinking phenol-functionalized hydrogels for cell encapsulation. Biomaterials Research, 20, 30. doi: 10.1186/s40824-016-0077-z

Lim, K. S., Schon, B. S., Mekhileri, N. V., Brown, G. C. J., Chia, C. M., Prabakar, S., Hooper, G. J., & Woodfield, T. B. F. (2016). New visible-light photoinitiating system for improved print fidelity in gelatin-based bioinks. ACS Biomaterials Science & Engineering, 2, 1752-1762. doi: 10.1021/acsbiomaterials.6b00149

Conference Contribution - Published proceedings: Abstract

Lim, K. S., Levato, R., Costa, P. F., Castilho, M. D., van Dorenmalen, K., Melchels, F. P. W., … Woodfield, T. B. F. (2016). Novel bio-resin for 3D bioprinting of complex cell-laden constructs with high spatial resolution via visible light photo-polymerisation and digital light processing. Proceedings of the International Conference on Biofabrication. OP-39. Retrieved from http://www.biofabrication2016.org/

Lim, K. S., Schon, B. S., Mekhileri, N. V., Hooper, G. J., & Woodfield, T. B. F. (2016). 3D bioprinting of gelatin constructs with enhanced shape fidelity and cell viability via visible light photo-polymerization. Proceedings of the International Conference on Biofabrication. OP-37. Retrieved from http://www.biofabrication2016.org/

Lim, K. S., Levato, R., Costa, P. F., Castilho, M., van Dorenmalen, K., Melchels, F. P. W., … Woodfield, T. B. F. (2016). Biofunctional and complex hydrogel architectures fabricated via visible light photo-crosslinking with digital light processing. European Cells & Materials, 31(Suppl. 1), (pp. 185). [Abstract]

Lim, K., Levato, R., Costa, P., Castilho, M., Dorenmalen, K., Melchels, F., … Woodfield, T. (2016). Biofabrication of hydrogel based "bio resins" via high resolution digital light processing. Proceedings of the 10th World Biomaterials Congress (WBC). (pp. 2691). Retrieved from http://www.wbc2016.org/

Brown, G. C., Lim, K. S., Farrugia, B. L., Hooper, G. J., & Woodfield, T. B. (2016). Fabrication of gelatin-heparin hydrogels with enhanced chondrogenic differentiation and tunable degradation. Frontiers in Bioengineering & Biotechnology, (WBC2016). doi: 10.3389/conf.FBIOE.2016.01.00613

Lim, K., Klotz, B., Brown, G., Melchels, F., Gawlitta, D., & Woodfield, T. (2016). Visible light polymerised hydrogels with improved cell survival and metabolic activity. Frontiers in Bioengineering & Biotechnology, (WBC2016). doi: 10.3389/conf.FBIOE.2016.01.01746

Conference Contribution - Poster Presentation (not in published proceedings)

Mutreja, I., Lim, K. S., Maradze, D., Liu, Y., Hooper, G., & Woodfield, T. B. F. (2016, August-September). Gelatin-magnesium carbonate nanocomposite hydrogels for improved osteogenic differentiation of mesenchymal stem cells. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Nelson, New Zealand.

Brown, G., Soliman, B., Bertlein, S., Lim, K., Hooper, G., Groll, J., & Woodfield, T. (2016, August-September). Covalent incorporation of heparin improves chondrogenic tissue formation in gelatin based hydrogels designed for cartilage tissue engineering. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Nelson, New Zealand.

Mekhileri, N. V., Schon, B., Lim, K. S., Mutreja, I., Hooper, G., & Woodfield, T. B. F. (2016, August-September). Bioassembly of complex multicellular tissues: An automated 3D microtissue assembly system for tissueengineering and high throughput screening. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Nelson, New Zealand.

Lim, K. S., Levato, R., Costa, P. F., Castilho, M. D., van Dorenmalen, K., Melchels, F. P. W., … Woodfield, T. B. F. (2016, August-September). Bioprinting of complex cell-encapsulated hydrogels with high spatial resolution via visible light photo-crosslinking and digital light processing. Poster session presented at the Queenstown Molecular Biology (QMB) Meetings, Nelson, New Zealand.

Soliman, B. G., Brown, G. C. J., Lim, K. S., & Woodfield, T. (2016, August). Photo-curable thiol-ene gelatin based hydrogels as bioinks for bioprinting. Poster session presented at the Medical Sciences Congress (MedSci), Nelson, New Zealand.

Conference Contribution - Verbal presentation and other Conference outputs

Lim, K. S., & Woodfield, T. B. F. (2016, August-September). An alternative visible light initiating system for 3D bioprinting in tissue engineering and regenerative medicine. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Nelson, New Zealand.

Woodfield, T. B. F., Lim, K., Tredinnick, S., Schon, B., Mekhileri, N., Brown, G., & Hooper, G. J. (2016, August-September). 3D bioprinting and bioassembly of tissue organoids for regeneration of cartilage and bone. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Nelson, New Zealand.

2015

Journal - Research Article

Lim, K. S., Ramaswamy, Y., Roberts, J. J., Alves, M.-H., Poole-Warren, L. A., & Martens, P. J. (2015). Promoting cell survival and proliferation in degradable poly(vinyl alcohol)-tyramine hydrogels. Macromolecular Bioscience, 15(10), 1423-1432. doi: 10.1002/mabi.201500121

Lim, K. S., Roberts, J. J., Alves, M.-H., Poole-Warren, L. A., & Martens, P. J. (2015). Understanding and tailoring the degradation of PVA-tyramine hydrogels. Journal of Applied Polymer Science, 132(26), 42142. doi: 10.1002/app.42142

Journal - Research Other

Lim, K. S., & Woodfield, T. B. F. (2015). Silk hydrogels for tissue engineering. Scripta-Ingenia, December(5), 3-7.

Conference Contribution - Published proceedings: Full paper

Aregueta-Robles, U. A., Lim, K. S., Martens, P. J., Lovell, N. H., Poole-Warren, L. A., & Green, R. (2015). Producing 3D neuronal networks in hydrogels for living bionic device interfaces. Proceedings of the Engineering in Medicine and Biology Society (EMBS) 37th Annual International Conference. (pp. 2600-2603). IEEE. doi: 10.1109/embc.2015.7318924

Lim, K. S., Ramaswamy, Y., Alves, M.-H., Green, R. A., Poole-Warren, L. A., & Martens, P. J. (2015). Optimization of crosslinking parameters for biosynthetic poly(vinyl-alcohol)-tyramine hydrogels. In D. A. Jaffray (Ed.), World Congress on Medical Physics and Biomedical Engineering (IFMBE Proceedings, Vol. 51). (pp. 284-287). Cham, Switzerland: Springer International. doi: 10.1007/978-3-319-19387-8_69

Conference Contribution - Published proceedings: Abstract

Brown, G., Lim, K., Woodfield, T., & Hooper, G. (2015). Strategies for temporal control of biophysical signals in biomimetic hydrogels for tissue engineering. Tissue Engineering Part A, 21(Suppl. 1), (pp. S-371-S-372). doi: 10.1089/ten.tea.2015.5000.abstracts

Lim, K. S., Brown, G. C., Mekhileri, N. V., Schon, B. S., Hooper, G. J., & Woodfield, T. B. (2015). The effect of oxygen inhibition in biofabrication of photocrosslinked hydrogels. Tissue Engineering Part A, 21(Suppl. 1), (pp. S-32). doi: 10.1089/ten.tea.2015.5000.abstracts

Conference Contribution - Poster Presentation (not in published proceedings)

Brown, G., Lim, K., Farrugia, B., Hooper, G., & Woodfield, T. (2015, November). Strategies for biofunctionalizing photocurable gelatin methacrylamide hydrogels for cartilage tissue engineering. Poster session presented at the International Conference on Biofabrication, Utrecht, The Netherlands.

Soliman, B., Klotz, B., Lim, K., Linssen, E., van Dorenmalen, K., Malda, J., … Gawlitta, D. (2015, November). Biofabrication of interconnected multi-scale microvessel networks within a gelatin methacrylamide hydrogel system for bone engineering. Poster session presented at the International Conference on Biofabrication, Utrecht, The Netherlands.

Conference Contribution - Verbal presentation and other Conference outputs

Lim, K. S., Mekhileri, N. V., Schon, B. S., Hooper, G. J., & Woodfield, T. B. F. (2015, May). Oxygen inhibition in 3D printing of photo-polymerised hydrogels. Verbal presentation at the International Symposium on Biointerface Science and Engineering: Joint Symposium of Biomaterials and Controlled Release Society (BCRS) Taiwan and the Australasian Society for Biomaterials and Tissue Engineering (ASBTE), Taipei, Taiwan.

Woodfield, T., Schon, B., Lim, K., Mekhileri, N., Schrobback, K., & Hooper, G. (2015, May). 3D printing and modular tissue assembly platforms for musculoskeletal regenerative medicine. Verbal presentation at the International Symposium on Biointerface Science and Engineering: Joint Symposium of Biomaterials and Controlled Release Society (BCRS) Taiwan and the Australasian Society for Biomaterials and Tissue Engineering (ASBTE), Taipei, Taiwan.

Lim, K. (2015, November). Finding the balance between oxygen inhibition and cell viability in biofabrication of photopolymerised hydrogels. Verbal presentation at the International Conference on Biofabrication, Utrecht, The Netherlands.

Lim, K. S., Mekhileri, N. V., Schon, B. S., Hooper, G. J., & Woodfield, T. B. F. (2015, May). Strategies to combat oxygen inhibition in 3D printing of photo-polymerised hydrogels. Verbal presentation at the 5th Asian Biomaterials Congress (ABMC5), Taipei, Taiwan.

Lindberg, G. C. J., Lim, K. S., Schon, B. S., Hooper, G. J., & Woodfield, T. B. F. (2015, April). Covalent incorporation of heparin in gelatin hydrogels for improved chondrocyte re-differentiation. Verbal presentation at the 5th International Symposium of Surface and Interface of Biomaterials (ISSIB) and the 24th Annual Conference of the Australasian Society for Biomaterials and Tissue Engineering (ASBTE), Sydney Australia.

Lim, K. S., Lindberg, G. C. J., Schon, B. S., Hooper, G. J., & Woodfield, T. B. F. (2015, April). Oxygen inhibition in 3D printing of photo-curable hydrogels. Verbal presentation at the 5th International Symposium of Surface and Interface of Biomaterials (ISSIB) and the 24th Annual Conference of the Australasian Society for Biomaterials and Tissue Engineering (ASBTE), Sydney Australia.

2014

Journal - Research Article

Peterson, M. B., Le-Masurier, S. P., Lim, K., Hook, J. M., Martens, P., & Granville, A. M. (2014). Incorporation of 5-hydroxyindazole into the self-polymerization of dopamine for novel polymer synthesis. Macromolecular Rapid Communications, 35(3), 291-297. doi: 10.1002/marc.201300746

Cheong, G. L. M., Lim, K. S., Jakubowicz, A., Martens, P. J., Poole-Warren, L. A., & Green, R. A. (2014). Conductive hydrogels with tailored bioactivity for implantable electrode coatings. Acta Biomaterialia, 10(3), 1216-1226. doi: 10.1016/j.actbio.2013.12.032

Conference Contribution - Published proceedings: Abstract

Lim, K., Alves, M.-H., Poole-Warren, L., & Martens, P. (2014). Improving cell survival in degradable biosynthetic PVA-Tyramine hydrogels: Incorporation of antioxidants. Proceedings of the Alliance for Design and Application in Tissue Engineering 5th Tissue Engineering Symposium: Tissue Engineering for Organ Regeneration. (pp. 49-50). ADATE. Retrieved from http://web.aeromech.usyd.edu.au/ADATE/TESymposium2014.html

Lindberg, G. C. J., Lim, K. S., & Woodfield, T. B. F. (2014). Strategy for fabricating cell-laden ECM derived hydrogels in cartilage tissue engineering. Proceedings of the Alliance for Design and Application in Tissue Engineering 5th Tissue Engineering Symposium: Tissue Engineering for Organ Regeneration. (pp. 33-34). ADATE. Retrieved from http://web.aeromech.usyd.edu.au/ADATE/TESymposium2014.html

Conference Contribution - Poster Presentation (not in published proceedings)

Lindberg, G., Lim, K., & Woodfield, T. (2014, September). Strategy for biofabricating cell-laden ECM derived hydrogels in cartilage tissue engineering. Poster session presented at the Division of Health Sciences Research Forum: Learning Different Research Languages, Dunedin, New Zealand.

2013

Journal - Research Article

Lim, K. S., Alves, M. H., Poole-Warren, L. A., & Martens, P. J. (2013). Covalent incorporation of non-chemically modified gelatin into degradable PVA-tyramine hydrogels. Biomaterials, 34(29), 7097-7105. doi: 10.1016/j.biomaterials.2013.06.005

Conference Contribution - Published proceedings: Full paper

Green, R. A., Lim, K. S., Henderson, W. C., Hassarati, R. T., Martens, P. J., Lovell, N. H., & Poole-Warren, L. A. (2013). Living electrodes: Tissue engineering in the neural interface. Proceedings of the 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBS). (pp. 6957-6960). IEEE. doi: 10.1109/EMBC.2013.6611158

2012

Journal - Research Article

Lim, K. S., Kundu, J., Reeves, A., Poole-Warren, L. A., Kundu, S. C., & Martens, P. J. (2012). The influence of silkworm species on cellular interactions with novel PVA/silk sericin hydrogels. Macromolecular Bioscience, 12(3), 322-332. doi: 10.1002/mabi.201100292