Investigating mitochondrial dysfunction and epigenetic changes in leukaemia

Overview

Acute myeloid leukaemia (AML) is a common blood cancer that is difficult to treat.

Markings on the DNA of AML cells (DNA methylation) are different to those of normal cells, and the patterns of these marks have an important role in how cancer develops and behaves. It is not clear what alters DNA patterning in leukaemia cells, and how this can be blocked and reversed.

We will study if malfunctioning mitochondria (tiny power stations in the cell) can change DNA markings in leukaemia. We will look at drugs that protect mitochondria to see whether they can help make leukaemia cells from patients act more like normal cells. We will also force mitochondrial dysfunction on healthy bone marrow stem cells while they are maturing and observe if they turn into leukaemia cells.

This research aims to reveal novel treatment strategies for leukaemia, and an opportunity to make a significant impact on cancer therapy and patient outcomes.

The student will acquire laboratory skills in:

• Cell culture
• DNA extraction
• Bisulfite conversions
• DNA methylation analysis
• Flow cytometry

Preferred student expertise

An interest in cancer and immunology and/or bioinformatics, cell culture experience an advantage but not required.

This is one of a number of projects on offer for the next intake of BBiomedSc(Hons) at the University of Otago, Christchurch campus.

Useful information

• Graduate research at the University of Otago
• Scholarships at the University of Otago

Further information

• University of Otago, Christchurch, BBiomedSc(Hons)
• Dr Annika Seddon’s profile page
• Mātai Hāora – Centre for Redox Biology and Medicine
• Department of Pathology and Biomedical Science
• Department of Pathology and Molecular Medicine, UOW