Professor Alexander McLellan

Immunologist

BSc, MSc (Canterbury) PhD (Otago)

Appointed: 2003

Email alex.mclellan@otago.ac.nz
Tel +64 3 479 7728

Teaching roles

HUBS 191 Human Body Systems 1
MELS 306 Medical Microbiology
MICN2/ELM2
MICR 223 Infection and Immunity
MICR 334 Advanced Immunology
MICR 462 Microbiology and Immunology Research
MICR 464 Medical Microbiology and Immunology
MICR 480 Research Project (Convenor)
MICR 490 Dissertation (Convenor)
MICR 495 / IMMU 495 Master's Thesis Preparation (Convenor)

Job description

Research

Pre-clinical testing of new CAR T cells and CAR NK cells in NSG mice. Building high-efficiency lentivirus, retrovirus and transposon gene transfer systems. Genetic designs for internal control of anti-cancer T cells and NK cells, including inducible promoters and bi-directional (dual CAR or CAR + payload) designs. Optimised expansion protocols for CAR NK cells, including kitsets for constructing artificial antigen presenting cells.

Teaching and research support

Graduate and undergraduate teaching portfolios in Immunology, Medicine, Medical Laboratory Science, Microbiology and Pharmaceutical Sciences, including lab-based teaching. Divisional Course Approver. Experience in Animal Ethics as Committee member for nine years. Current Chair of Bioimager Acquisition Committee and former Chair of Flow Cytometry facility.

Research interests

Cancer immunotherapy

Current research

CAR T cells (Chimeric Antigen Receptor-modified T cells) against solid tumours. Dual CAR T cells using bi-directional promoters, tumour-micro-environment sensors and CAR T cell metabolism boosting with coding and non-coding RNA. Cytokine and chemokine receptor strategies to improve T cell function against solid tumours.

Research projects

Chimeric Antigen Receptor T cells (CAR-T cells) and NK cells targeting solid tumours
Dual CAR constructs against B cell malignancies and solid tumours using bi-directional promoters
Sleeping Beauty and Retroviral vector constructs utilising drug and auto-inducible-promoters
Targeting the tumour microenvironment
Fighting the cytokine storm
Improved anti-cancer T cell migration using targeted cytokines

A photo of the Mclellan Lab 2020

Mclellan Lab 2020

Lab Group

A profile photo of Professor Alexander McLellan

Professor Alex Mclellan
Principal Investigator
Email alex.mclellan@otago.ac.nz
Tel +64 3 479 7728

Staff

Dr Sarah Saunderson – Teaching Fellow
TBA – Post Doctoral Fellow
Thiloma Liyanage – Assistant Research Fellow

Postgraduate student

Sam Smith-Bell – PhD Student
Lachie Dobson – PhD student
Tom McDermott – BSc(Hons) GENE
Blake Sluyter – BSc(Hons)

Current Students

Lachian Dobson (PhD, Bi-directional expression in CAR NK cells
Sam Smith-Bell (PhD), In vivo control of CAR T cell activity
Tom McDermott BSc (Hons), Inducible bi-directional expression in CAR T cells
Blake Sluyter BSc(Hons), NK CAR cell recognition and killing mechanisms

Staff

Dr Sarah Saunderson (BBiomedSc(Hons), PhD; Research Fellow): Marsden Fast Start funded
Dr Josh Halpin (Post Doctoral Fellow, HRC Project Grant)
Liz Berry Research coordinator
Professor Alex McLellan (BSc, MSc, PhD; Chef): Tea and coffee synthesis, grant applications and guidance

Recently completed

Josh Halpin (PhD 2023)
Lydia White (MSc 2022)
Lachlan Dobson (BBiomedSc Hons 2022)
Courtney Leith (PGDipSci 2022)
Madeleine O'Leary (BScHons 2022)
Jayde Ngata BSc(Hons) 2021
Seyed Ali Hosseini Rad PhD 2020
Grace Tan PhD 2021
Aarati Poudel PhD 2021
Ali Hosseini PhD 2020
Lydia White BSc(Hons) 2020
Palak Shrivastava BSc(Hons) 2020
Kevin He PGDipSci 2018, MSc 2020.
Sam Smith-Bell BBiomedSc Hons 2019
Ella Dally BBiomedSc Hons 2019
Jessica Matheson PGDipSci 2019
Thu Dinh BBiomedSci (Hons) 2018
Thomas Marsland BSc(Hons; with A/Prof. Mik Black) 2018
Josh Halpin BSc(Hons) 2018
Hannah Goy BSc(Hons) 2017
Jessica Bruce-Jones BSc(Hons) 2016
Bailey Kennedy BSc(Hons) 2015

Other activities

Member of the Centre for Translational Cancer Research (Otago) and The Renal Theme (Otago). Former Manager, University of Otago Flow Cytometry Facility (2004-2012)

Current collaborations

Dr. Yoshio Nakatani; Dept. Microbiology and Immunology (From the stairwell to the world: 2017 Proof of Concept winners named)
Associate Professor. Mike Kershaw and Professor Phil Darcy; Peter MacCallum Cancer Centre, Melbourne, Australia
Professor Mette Rosenkilde / Dr Stefanie Karlshøj; University of Copenhagen, Denmark): GPCR and the tumour microenvironment in T cell immunotherapy

Sources of funding

The group has recently received funding from:
HRC (2020 and 2017 Explorer grant)
The Royal Society Marsden Fund (2019)
The NZ Cancer Society 2018
The Proof of Concept Award (2017; cytokine storm and tumour immunotherapy)
The William Sherriff Trust
Lottery Health Research
The University of Otago Research Grant committee
Otago School of Medical Sciences. In 2014, a generous donation of $4500 from Audrey Tay's 'Shave for Cancer' supported our work on cancer-related thrombosis – thanks Audrey!

Recent publicity

Way to make cancer treatment safer wins Otago innovation competition

Publications

Saunderson, S. C., Halpin, J. C., Tan, G. M. Y., Shrivastava, P., & McLellan, A. D. (2024). Conversion of anti-tissue factor antibody sequences to chimeric antigen receptor and bi-specific T-cell engager format. Cancer Immunology, Immunotherapy, 73, 195. doi: 10.1007/s00262-024-03778-3 Journal - Research Article

Khaniya, A., Hosseini Rad, S. M. A., Halpin, J., Tawinwung, S., McLellan, A., Suppipat, K., & Hirankarn, N. (2024). Development of a compact bidirectional promoter-driven dual chimeric antigen receptor (CAR) construct targeting CD19 and CD20 in the Sleeping Beauty (SB) transposon system. Journal for ImmunoTherapy of Cancer, 12, e008555. doi: 10.1136/jitc-2023-008555 Journal - Research Article

Rad S M, A. H., Wannigama, D. L., Hirankarn, N., & McLellan, A. D. (2023). The impact of non-synonymous mutations on miRNA binding sites within the SARS-CoV-2 NSP3 and NSP4 genes. Scientific Reports, 13, 16945. doi: 10.1038/s41598-023-44219-y Journal - Research Article

McLellan, A. (2023, April). Passengers in blood and skin: Natural and engineered immune cells. University of Otago, Dunedin, New Zealand. [Inaugural Professorial Lecture]. Other Research Output

Dobson, L. J., Saunderson, S. C., Smith-Bell, S. W. J., & McLellan, A. D. (2023). Sleeping Beauty kit sets provide rapid and accessible generation of artificial antigen-presenting cells for natural killer cell expansion. Immunology & Cell Biology. Advance online publication. doi: 10.1111/imcb.12679 Journal - Research Article

2024

Journal - Research Article

2023

Journal - Research Article

Conference Contribution - Published proceedings: Abstract

Bull, D., Hazlett, J., McLellan, A., & Dunbier, A. (2023). Development and characterisation of immunocompetent models for metastatic oestrogen receptor positive breast cancer. Molecular Oncology, 17(Suppl. 1), (pp. 513-514). doi: 10.1002/1878-0261.13471

Other Research Output

McLellan, A. (2023, April). Passengers in blood and skin: Natural and engineered immune cells. University of Otago, Dunedin, New Zealand. [Inaugural Professorial Lecture].

2022

Journal - Research Article

Tan, G. M. Y., Poudel, A., Hosseini Rad, S. M. A., & McLellan, A. D. (2022). Anti-apoptotic c-FLIP reduces the anti-tumour activity of chimeric antigen receptor T cells. Cancers, 14(19), 4854. doi: 10.3390/cancers14194854

Journal - Research Other

Saunderson, S. C., Hosseini Rad, S. M. A., & McLellan, A. D. (2022). Noise-reduction and sensitivity-enhancement of a Sleeping Beauty-based Tet-On system [Communication]. Genes, 13, 1679. doi: 10.3390/genes13101679

Hosseini Rad, S. M. A., Halpin, J. C., Tawinwung, S., Suppipat, K., Hirankarn, N., & McLellan, A. D. (2022). MicroRNA-mediated metabolic reprogramming of chimeric antigen receptor T cells. Immunology & Cell Biology, 100, 424-439. doi: 10.1111/imcb.12551

White, L. G., Goy, H. E., Rose, A. J., & McLellan, A. D. (2022). Controlling cell trafficking: Addressing failures in CAR T and NK cell therapy of solid tumours. Cancers, 14, 978. doi: 10.3390/cancers14040978

Conference Contribution - Published proceedings: Abstract

Bull, D., Hazlett, J., McLellan, A., & Dunbier, A. (2022). Investigating the role of immunotherapy in the treatment of metastatic oestrogen receptor positive breast cancer. Proceedings of the Genetics Otago (GO) Annual Symposium. Retrieved from https://blogs.otago.ac.nz/go

White, L., Halpin, J., Leith, C., Poudel, A., Saunderson, S., Jackson, S., Fagerlund, R., Fineran, P., & McLellan, A. (2022). Exploiting the intrinsic insolubility of T cell and NK cell-activating cytokines and chemokines for Escherichia coli expression. Proceedings of the New Zealand Branch of the Australian and New Zealand Society for Immunology (NZASI) Conference. (pp. 45). Retrieved from https://nzasi2022.com/programme

Dobson, L., Goy, H., McLellan, A., & Saunderson, S. (2022). Sleeping beauty transposon-based vector kit-sets for the manufacture of artificial antigen presenting cells for CAR NK or T cell therapy. Proceedings of the New Zealand Branch of the Australian and New Zealand Society for Immunology (NZASI) Conference. (pp. 31). Retrieved from https://nzasi2022.com/programme

Halpin, J., Goy, H., Smith-Bell, S., Hosseini Rad, A., Saunderson, S., & McLellan, A. (2022). Drug and auto-inducible promoters to improve CAR T cell efficacy and patient safety in solid tumour treatment. Proceedings of the New Zealand Branch of the Australian and New Zealand Society for Immunology (NZASI) Conference. (pp. 14). Retrieved from https://nzasi2022.com/programme

Conference Contribution - Verbal presentation and other Conference outputs

McLellan, A. (2022, August). Promoters to drive CAR T cells persistence and activity. Verbal presentation at the Cancer Satellite Meeting: Queenstown Research Week, Queenstown, New Zealand.

2021

Journal - Research Other

Rad S M, A. H., Halpin, J. C., Mollaei, M., Smith Bell, S. W. J., Hirankarn, N., & McLellan, A. D. (2021). Metabolic and mitochondrial functioning in chimeric antigen receptor (CAR)-T cells. Cancers, 13, 1229. doi: 10.3390/cancers13061229

Conference Contribution - Published proceedings: Abstract

Bull, D., Hazlett, J., McLellan, A., & Dunbier, A. (2021). Investigating the role of immunotherapy in the treatment of metastatic oestrogen receptor positive breast cancer. Proceedings of the Genetics Otago (GO) Annual Symposium. Retrieved from https://blogs.otago.ac.nz/go

2020

Journal - Research Article

He, K., Hosseini Rad, S. M. A., Poudel, A., & McLellan, A. D. (2020). Compact bidirectional promoters for dual-gene expression in a sleeping beauty transposon. International Journal of Molecular Sciences, 21, 9256. doi: 10.3390/ijms21239256

Hosseini Rad, S. M. A., Tan, G. M. Y., Poudel, A., He, K., & McLellan, A. D. (2020). Regulation of human Mcl-1 by a divergently-expressed antisense transcript. Gene, 762, 145016. doi: 10.1016/j.gene.2020.145016

Hosseini Rad, S. M. A., Poudel, A., Tan, G. M. Y., & McLellan, A. D. (2020). Optimisation of Tet-On inducible systems for Sleeping Beauty-based chimeric antigen receptor (CAR) applications. Scientific Reports, 10, 13125. doi: 10.1038/s41598-020-70022-0

Rad S M, A. H., Poudel, A., Tan, G. M. Y., & McLellan, A. D. (2020). Promoter choice: Who should drive the CAR in T cells? PLoS ONE, 15(7), e0232915. doi: 10.1371/journal.pone.0232915

Rad S M, A. H., & McLellan, A. D. (2020). Implications of SARS-CoV-2 mutations for genomic RNA structure and host microRNA targeting. International Journal of Molecular Sciences, 21, 4807. doi: 10.3390/ijms21134807

Conference Contribution - Published proceedings: Abstract

Poudel, A., Rad, S. M., Tan, G., & McLellan, A. D. (2020). Enhancing T cell function for cancer immunotherapy by microRNA mediated knockdown of PRKAR1A. Journal for ImmunoTherapy of Cancer, 8(Suppl. 2), (pp. A43). doi: 10.1136/jitc-2020-ITOC7.83

Hosseini Rad, A., Tan, G. M. Y., Poudel, A., & McLellan, A. (2020). Enhancing CAR T cell persistence and memory through modulating mitochondrial function. Journal for ImmunoTherapy of Cancer, 8(Suppl 2), (pp. A42). doi: 10.1136/jitc-2020-ITOC7.81

Tan, G. M. Y., Hosseini, S. M. A. R., Poudel, A., & McLellan, A. (2020). Expression of anti-apoptotic gene cFLIP to enhance persistence in CAR T cells. Journal for ImmunoTherapy of Cancer, 8(Suppl. 2), (pp. A6). doi: 10.1136/jitc-2020-ITOC7.11

2019

Journal - Research Other

Weinkove, R., George, P., Dasyam, N., & McLellan, A. D. (2019). Selecting costimulatory domains for chimeric antigen receptors: Functional and clinical considerations. Clinical & Translational Immunology, 8(5), e1049. doi: 10.1002/cti2.1049

McLellan, A. D., & Hosseini Rad, S. M. A. (2019). Chimeric antigen receptor T cell persistence and memory cell formation. Immunology & Cell Biology, 97, 664-674. doi: 10.1111/imcb.12254

Conference Contribution - Published proceedings: Abstract

Budhwani, M., McLellan, A., Kleffmann, T., & Hibma, M. (2019). Extracellular vesicles released from human papillomavirus type 16 E7 keratinocytes suppress antigen presenting cell activation and antigen-specific T cell proliferation. European Journal of Immunology, 49(Suppl. 2), (pp. 7-8). doi: 10.1002/eji.201970200

Conference Contribution - Verbal presentation and other Conference outputs

McLellan, A. D., Poudel, A., Tan, G. M. Y., Halpin, J. C., Smith-Bell, S. W. J., Dally, E. R., Laws, G. A., Matheson, J. A., He, K., Saunderson, S. C., & Hosseini Rad, S. M. A. (2019, August-September). Promoters to drive Chimeric Antigen Receptor (CAR) T cell therapy. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

2018

Journal - Research Other

Muhsin-Sharafaldine, M.-R., & McLellan, A. D. (2018). Apoptotic vesicles: Deathly players in cancer-associated coagulation. Immunology & Cell Biology, 96(7), 723-732. doi: 10.1111/imcb.12162

Muhsin-Sharafaldine, M.-R., & McLellan, A. D. (2018). Tumor-derived apoptotic vesicles: With death they do part. Frontiers in Immunology, 9, 957. doi: 10.3389/fimmu.2018.00957

Conference Contribution - Verbal presentation and other Conference outputs

McLellan, A. D., Hosseini Rad, S. M. A., Muhsin-Sharafaldine, M.-R., Poudel, A., Tan, G. M. Y., Steens, J., Halpin, J. C., Matheson, J. A., He, K., Goy, H. E., Dinh, T. N., Haw, R. T., & Saunderson, S. C. (2018, August). Engineering memory, survival and safety into chimeric antigen receptor (CAR) T cells. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Queenstown, New Zealand.

2017

Journal - Research Article

Saunderson, S. C., & McLellan, A. D. (2017). Role of lymphocyte subsets in the immune response to primary B cell-derived exosomes. Journal of Immunology, 199(7), 2225-2235. doi: 10.4049/jimmunol.1601537

Muhsin-Sharafaldine, M.-R., Saunderson, S. C., Dunn, A. C., & McLellan, A. D. (2017). Melanoma growth and lymph node metastasis is independent of host CD169 expression. Biochemical & Biophysical Research Communications, 486(4), 965-970. doi: 10.1016/j.bbrc.2017.03.138

Muhsin-Sharafaldine, M.-R., Kennedy, B. R., Saunderson, S. C., Buchanan, C. R., Dunn, A. C., Faed, J. M., & McLellan, A. D. (2017). Mechanistic insight into the procoagulant activity of tumor-derived apoptotic vesicles. Biochimica et Biophysica Acta: General Subjects, 1861(2), 286-295. doi: 10.1016/j.bbagen.2016.11.020

Conference Contribution - Verbal presentation and other Conference outputs

Poudel, A., Saunderson, S. C., Karlshøj, S., Rosenkilde, M., & McLellan, A. D. (2017, July). Enhancing the migration and activation of anti-cancer T-cells through chimeric G-protein coupled receptors. Verbal presentation at the New Zealand Branch of the Australasian Society for Immunology (NZ ASI) Annual Scientific Meeting, Christchurch, New Zealand.

Saunderson, S. C., & McLellan, A. D. (2017, July). Using genetically modified T cells to target a factor expressed on differentiated, solid tumours. Verbal presentation at the New Zealand Branch of the Australasian Society for Immunology (NZ ASI) Annual Scientific Meeting, Christchurch, New Zealand.

Hosseini Rad, S. M. A., Saunderson, S. C., & McLellan, A. D. (2017, July). Enhancing chimeric antigen receptor (CAR) T cell persistence and memory through small activating RNA expressed in CAR constructs. Verbal presentation at the New Zealand Branch of the Australasian Society for Immunology (NZ ASI) Annual Scientific Meeting, Christchurch, New Zealand.

Haw, R. T. Y., Saunderson, S. C., & McLellan, A. D. (2017, July). Cancer stem cells: A novel therapeutic target for chimeric antigen receptor T cells? Verbal presentation at the New Zealand Branch of the Australasian Society for Immunology (NZ ASI) Annual Scientific Meeting, Christchurch, New Zealand.

2016

Journal - Research Article

Jemon, K., Leong, C.-M., Ly, K., Young, S. L., McLellan, A. D., & Hibma, M. H. (2016). Suppression of the CD8 T cell response by human papillomavirus type 16 E7 occurs in Langerhans cell-depleted mice. Scientific Reports, 6, 34789. doi: 10.1038/srep34789

Muhsin-Sharafaldine, M.-R., Saunderson, S. C., Dunn, A. C., Faed, J. M., Kleffmann, T., & McLellan, A. D. (2016). Procoagulant and immunogenic properties of melanoma exosomes, microvesicles and apoptotic vesicles. Oncotarget, 7(35), 56279-56294. doi: 10.18632/oncotarget.10783

Black, L. V. C., Saunderson, S. C., Coutinho, F. P., Muhsin-Sharafaldine, M.-R., Damani, T. T., Dunn, A. C., & McLellan, A. D. (2016). The CD169 sialoadhesin molecule mediates cytotoxic T-cell responses to tumour apoptotic vesicles. Immunology & Cell Biology, 94, 430-438. doi: 10.1038/icb.2015.111

Conference Contribution - Published proceedings: Abstract

Saunderson, S. C., & McLellan, A. D. (2016). A critical role for NK cells in exosome-induced CD-8⁺ T cell cytotoxicity. European Journal of Immunology, 46(Suppl. 1), (pp. 1145). doi: 10.1002/eji.201670200

Muhsin-Sharafaldine, M.-R., Saunderson, S. C., Dunn, A. C., & McLellan, A. D. (2016). The role of CD169 in tumour metastasis. European Journal of Immunology, 46(Suppl. 1), (pp. 1094). doi: 10.1002/eji.201670200

Muhsin-Sharafaldine, M.-R., Saunderson, S. C., Dunn, A. C., & McLellan, A. D. (2016). Role of melanoma apoptotic vesicles in tumour immunity and coagulation. European Journal of Immunology, 46(Suppl. 1), (pp. 891). doi: 10.1002/eji.201670200

Conference Contribution - Verbal presentation and other Conference outputs

Muhsin-Sharafaldine, M.-R., Saunderson, S. C., Dunn, A. C., Faed, J. M., Kleffmann, T., & McLellan, A. D. (2016, August-September). Procoagulant and immunogenic properties of tumour exosomes, microvesicles and apoptotic vesicles. Verbal presentation at the Queenstown Molecular Biology (QMB) Meetings, Nelson, New Zealand.

2015

Conference Contribution - Poster Presentation (not in published proceedings)

Muhsin, M.-R., Dunn, A., Saunderson, S., Faed, J., & McLellan, A. (2015, October). The effects of tumour vesicles on cancer metastasis. Poster session presented at the Otago Spotlight Series: Cancer Research, Wellington, New Zealand.

2014

Journal - Research Article

Dunn, E. A., Roxburgh, M., Larsen, L., Smith, R. A. J., McLellan, A. D., Heikal, A., Murphy, M. P., & Cook, G. M. (2014). Incorporation of triphenylphosphonium functionality improves the inhibitory properties of phenothiazine derivatives in Mycobacterium tuberculosis. Bioorganic & Medicinal Chemistry, 22(19), 5320-5328. doi: 10.1016/j.bmc.2014.07.050

McLellan, A. D. (2014). A critical role for natural killer cells in dendritic cellbased anticancer immunotherapy. OncoImmunology, 3(4), e28582. doi: 10.4161/onci.28582

Tannock, G. W., Taylor, C., Lawley, B., Loach, D., Gould, M., Dunn, A. C., McLellan, A. D., Black, M. A., McNoe, L., Dekker, J., … Collett, M. A. (2014). Altered transcription of murine genes induced in the small bowel by administration of probiotic strain Lactobacillus rhamnosus HN001. Applied & Environmental Microbiology, 80(9), 2851-2859. doi: 10.1128/aem.00336-14

Bouwer, A. L., Saunderson, S. C., Caldwell, F. J., Damani, T. T., Pelham, S. J., Dunn, A. C., Jack, R. W., … McLellan, A. D. (2014). NK cells are required for dendritic cell-based immunotherapy at the time of tumor challenge. Journal of Immunology, 192(5), 2514-2521. doi: 10.4049/jimmunol.1202797

Saunderson, S. C., Dunn, A. C., Crocker, P. R., & McLellan, A. D. (2014). CD169 mediates the capture of exosomes in spleen and lymph node. Blood, 13(2), 208-216. doi: 10.1182/blood-2013-03-489732

Conference Contribution - Verbal presentation and other Conference outputs

McLellan, A. (2014, July). Extracellular vesicles: Immunity and coagulation. Verbal presentation at the Oxygen Theme Meeting, Christchurch, New Zealand.

2013

Journal - Research Article

Bouwer, A. L., Saunderson, S. C., Dunn, A. C., Lester, K. L., Crowley, L. R., Jack, R. W., & McLellan, A. D. (2013). Rapid interferon-gamma release from natural killer cells induced by a streptococcal commensal. Journal of Interferon & Cytokine Research, 33(8), 459-466. doi: 10.1089/jir.2012.0116

Czepluch, W., Dunn, A. C., Everitt, C. L., Dorer, D., Saunderson, S. C., Aldwell, F. E., & McLellan, A. D. (2013). Extracellular forms of Mycobacterium bovis BCG in the mucosal lymphatic tissues following oral vaccination. International Journal of Mycobacteriology, 2(1), 44-50. doi: 10.1016/j.ijmyco.2012.11.004

Conference Contribution - Published proceedings: Abstract

Muhsin, M.-R., Saunderson, S., Black, L., Dunn, A., Faed, J., & McLellan, A. (2013). The effects of tumour vesicles on cancer progression. Proceedings of the 43rd Australasian Society for Immunology (ASI) Annual Scientific Meeting. (pp. 137-138). Retrieved from http://www.asi2013.org/

Saunderson, S. C., Crocker, P. R., & McLellan, A. D. (2013). CD169 mediates the capture of exosomes in spleen and lymph node. Proceedings of the 43rd Australasian Society for Immunology (ASI) Annual Scientific Meeting. (pp. 71). Retrieved from http://www.asi2013.org/

McLellan, A. (2013). Role of sialic acids in particulate antigen entry into lymphatic organs. Proceedings of the 43rd Australasian Society for Immunology (ASI) Annual Scientific Meeting. (pp. 59). Retrieved from http://www.asi2013.org/

Conference Contribution - Poster Presentation (not in published proceedings)

Muhsin, R., Saunderson, S. C., Black, L. V. C., Dunn, A. C., & McLellan, A. (2013, September). The effects of tumour vesicles on cancer progression. Poster session presented at the Division of Health Sciences Research Forum: Foundations for Health: Otago Health Research, Christchurch, New Zealand.

2012

Journal - Research Article

Ting, Y.-T., Coates, P. T., Walker, R. J., & McLellan, A. D. (2012). Urinary tubular biomarkers as potential early predictors of renal allograft rejection. Nephrology, 17(1), 11-16. doi: 10.1111/j.1440-1797.2011.01536.x

2011

Journal - Research Article

Baker, N. J., Schofield, J. C., Caswell, M. D., & McLellan, A. D. (2011). Effects of early atipamezole reversal of medetomidine—ketamine anesthesia in mice. Journal of the American Association for Laboratory Animal Science, 50(6), 916-920.

Nixon, G., Blattner, K., Dawson, J., Dovey, S., Black, M. A., Wilkins, G., Dunn, A. C., & McLellan, A. D. (2011). Streptokinase antibodies in patients presenting with acute coronary syndrome in three rural New Zealand populations. Journal of Clinical Pathology, 64, 426-429. doi: 10.1136/jcp.2010.085837

2010

Chapter in Book - Research

Stoitzner, P., Romani, N., McLellan, A. D., Tripp, C. H., & Ebner, S. (2010). Isolation of skin dentritic cells from mouse and man. In S. H. Naik (Ed.), Dendritic cell protocols: Methods in Molecular Biology (Vol. 595). (2nd ed.) (pp. 235-248). Totowa, NJ: Humana Press. doi: 10.1007/978-1-60761-421-0_16

Journal - Research Article

Ting, Y. T., Coates, T. P., Marti, H. P., Dunn, A. C., Parker, R. M., Pickering, J. W., Jack, R. W., Kemp, R. A., Walker, R. J., & McLellan, A. D. (2010). Urinary soluble HLA-DR is a potential biomarker for acute renal transplant rejection. Transplantation, 89(9), 1071-1078. doi: 10.1097/TP.0b013e3181d15492

Temme, S., Eis-Hübinger, A. M., McLellan, A. D., & Koch, N. (2010). The herpes simplex virus-1 encoded glycoprotein B diverts HLA-DR into the exosome pathway. Journal of Immunology, 184(1), 236-243. doi: 10.4049/jimmunol.0902192

Bouwer, A. L., Netter, P., Kemp, R. A., & McLellan, A. D. (2010). A defined serum-free medium useful for monitoring anti-melanoma responses induced by dendritic cell immunotherapy. Journal of Immunological Methods, 352, 178-181. doi: 10.1016/j.jim.2009.11.001

Conference Contribution - Verbal presentation and other Conference outputs

McLellan, A. (2010, September). From piddle-pot to bench to bedside: A urine test for renal transplant rejection. Verbal presentation at the Fourth Annual Division of Health Sciences Research Forum, Dunedin, New Zealand.

Other Research Output

McCall, J., Walker, R. & McLellan, A. (2010, April). The immune system and organ transplantation. World Day of Immunology Public Lecture, Dunedin, New Zealand. [Public Seminar].

2009

Journal - Research Article

Ting, Y. T., Temme, S., Koch, N., & McLellan, A. D. (2009). A new monoclonal antibody recognizing a linear determinant on the HLA-DRα chain N-terminus. Hybridoma, 28(6), 423-429. doi: 10.1089/hyb.2009.0050

Journal - Research Other

McLellan, A. D. (2009). Monoclonal antibody 1C4.6 against the HLA DRα N-terminus. Hybridoma, 28(6), 468. doi: 10.1089/hyb.2009.0062.MAb

McLellan, A. D. (2009). Exosome release by primary B cells. Critical Reviews in Immunology, 29(3), 203-217.

Conference Contribution - Published proceedings: Abstract

Czepluch, W., Everitt, C. L., Cook, G. M., & McLellan, A. D. (2009). Role of soluble factors released by coccoid forms of mycobacteria in the immune response. Proceedings of the Australasian Society for Immunology 39th Annual Scientific Meeting. ASI. Retrieved from http://www.asi2009.org/

Ting, Y., Coates, P. T., Marti, H., Dunn, A. C., Parker, R. M., Jack, R. W., Kemp, R. A., Walker, R. J., & McLellan, A. D. (2009). Urinary soluble HLA-DR is a potential biomarker for acute renal transplant rejection. Proceedings of the Australasian Society for Immunology 39th Annual Scientific Meeting. ASI. Retrieved from http://www.asi2009.org/

Saunderson, S. C., Kemp, R. A., & McLellan, A. D. (2009). CD169 (sialoadhesin) mediates sialic acid-dependent binding of exosomes to marginal zone metallophilic macrophages and subcapsular sinus macrophages. Proceedings of the Australasian Society for Immunology 39th Annual Scientific Meeting. ASI. Retrieved from http://www.asi2009.org/

Bouwer, A. L., Stoitzner, P., Kemp, R. A., & McLellan, A. D. (2009). Natural killer cells activate CD8⁺ T cells at both the priming and challenge phases of an anti-tumour response. Proceedings of the Australasian Society for Immunology 39th Annual Scientific Meeting. ASI. Retrieved from http://www.asi2009.org/

Conference Contribution - Verbal presentation and other Conference outputs

Bouwer, A. L., Stoitzner, P., & McLellan, A. D. (2009, June). Optimised activation of NK cells: Application to tumour immunotherapy. Verbal presentation at the New Zealand Australasian Society for Immunology (NZ ASI) Branch Meeting, Wellington, New Zealand.

Ting, Y.-T., Walker, R., Coates, P. T., Dunn, A. C., Parker, R., Jack, R. W., … McLellan, A. D. (2009, June). Soluble HLA-DR: A potential urinary biomarker for renal transplant rejection. Verbal presentation at the New Zealand Australasian Society for Immunology (NZ ASI) Branch Meeting, Wellington, New Zealand.

Saunderson, S. C., Schuberth, P., Dunn, A. C., Miller, L., Hock, B. D., MacKay, P. A., … Jack, R. W., & McLellan, A. D. (2009, June). Primary B cell derived exosomes bind the marginal zone of the spleen. Verbal presentation at the New Zealand Australasian Society for Immunology (NZ ASI) Branch Meeting, Wellington, New Zealand.

Everitt, C. L., Czepluch, W., Dunn, A. C., & McLellan, A. D. (2009, June). The role of coccoid forms of mycobacteria and subcapsular macrophages in immunity. Verbal presentation at the New Zealand Australasian Society for Immunology (NZ ASI) Branch Meeting, Wellington, New Zealand.

2008

Journal - Research Article

Gebhard, S., Hümpel, A., McLellan, A. D., & Cook, G. M. (2008). The alternative sigma factor SigF of Mycobacterium smegmatis is required for survival of heat shock, acidic pH and oxidative stress. Microbiology, 154(Pt 9), 2786-2795. doi: 10.1099/mic.0.2008/018044-0

Saunderson, S. C., Schuberth, P. C., Dunn, A. C., Miller, L., Hock, B. D., MacKay, P. A., … Jack, R. W., & McLellan, A. D. (2008). Induction of exosome release in primary B cells stimulated via CD40 and the IL-4 receptor. Journal of Immunology, 180(12), 8146-8152.

Conference Contribution - Poster Presentation (not in published proceedings)

Saunderson, S. C., Schuberth, P. C., Dunn, A. C., Miller, L., Hock, B. D., MacKay, P. A., … Jack, R. W., & McLellan, A. D. (2008, November). Induction of exosome release in primary B cells stimulated via CD40 and the IL-4 receptor. Poster session presented at the Medical Sciences Congress, Queenstown, New Zealand.

Ting, Y.-T., Dunn, A. C., Parker, R., Jack, R. W., Berney-Meyer, L., Walker, R., & McLellan, A. D. (2008, August-September). Biochemical characterization of soluble HLA-DR: A potential urinary biomarker for renal transplant rejection. Poster session presented at the Queenstown Molecular Biology Meetings, Queenstown, New Zealand.

Conference Contribution - Verbal presentation and other Conference outputs

Czepluch, W., Lambeth, M. R., Aldwell, F. E., & McLellan, A. D. (2008, November). The extracellular lifestyle of Mycobacterium bovis BCG in draining lymph nodes. Verbal presentation at the Medical Sciences Congress, Queenstown, New Zealand.

Bouwer, A. L., Stoitzner, P., Tagg, J. R., Jack, R. W., & McLellan, A. D. (2008, November). Role of endogenous NK cells in tumour immunotherapy. Verbal presentation at the Medical Sciences Congress, Queenstown, New Zealand.

2007

Journal - Research Article

Koch, N., McLellan, A. D., & Neumann, J. (2007). A revised model for invariant chain-mediated assembly of MHC class II peptide receptors. Trends in Biochemical Sciences, 32(12), 532-537.

Dorer, D. E., Czepluch, W., Lambeth, M. R., Dunn, A. C., Reitinger, C., Aldwell, F. E., & McLellan, A. D. (2007). Lymphatic tracing and T cell responses following oral vaccination with live Mycobacterium bovis (BCG). Cellular Microbiology, 9(2), 544-553.

O'Donnell, J. L., Hayman, M. W., Spellerberg, M. B., McLellan, A. D., Brooksbank, K., Chapman, P. T., & Stamp, L. K. (2007). Antineutrophil cytoplasmic antibody measurement: Advantages and disadvantages of a capture PR3 ELISA and a direct PR3 ELISA. Pathology, 39(2), 258-263.

2006

Journal - Research Article

MacKay, P. A., LeibundGut-Landmann, S., Koch, N., Dunn, A. C., Reith, W., Jack, R. W., & McLellan, A. D. (2006). Circulating, soluble forms of major histocompatability complex antigens are not exosome-associated. European Journal of Immunology, 36, 2875-2884.

Conference Contribution - Published proceedings: Abstract

Bouwer, A., Jack, R., Crowley, L., Tagg, J., & McLellan, A. (2006). Dendritic cells stimulated by Gram-positive bacteria induce potent interferon-gamma release from natural killer cells in a contact- and Interleukin-12/IL-18-dependent manner. New Zealand Medical Journal, 119(1245). Retrieved from http://journal.nzma.org.nz/journal/119-1245/2331/content.pdf

Hayman, M. W., Spellerberg, M. B., Brookshank, K., McLellan, A., Chapman, P. T., Stamp, L. K., & O'Donnell, J. L. (2006). Antineutrophil cytoplasmic antibody measurement by capture PR3 Elisa: Advantages and disadvantages. Annals of the Rheumatic Diseases, 65(Supp. II), (pp. 370-371). [Abstract]

Hayman, M. W., van Beijnen, M. T. A., Spellerberg, M. B., McLellan, A., Stamp, L. K., & O'Donnell, J. L. (2006). Soluable human leukocyte antigen as a diagnostic marker for rhematoid arthritis. Annals of the Rheumatic Diseases, 65(Suppl. II), (pp. 585). [Abstract]

2005

Journal - Research Article

Douillard, P., Stoitzner, P., Tripp, C. H., Clair-Moninot, V., Ait-Yahia, S., McLellan, A. D., … Saeland, S. (2005). Mouse lymphoid tissu contains distinct subsets of langerin/CD207⁺ dendritic cells, only one of which represents epidermal-derived langerhans cells. Journal of Investigative Dermatology, 125, 983-994.

Hock, B. D., Roberts, G., McKenzie, J. L., Gokhale, P., Salm, N., McLellan, A. D., Patton, N. W., & Roake, J. A. (2005). Exposure to the electrofusion process can increase the immunogenicity of human cells. Cancer Immunology, Immunotherapy, 54, 880-890.

Aldwell, F. E., Baird, M. A., Fitzpatrick, C. E., McLellan, A. D., Cross, M. L., Lambeth, M. R., & Buchan, G. S. (2005). Oral vaccination of mice with lipid-encapsulated Mycobacterium bovis BCG: Anatomical sites of bacterial replication and immune activity [Brief communication]. Immunology & Cell Biology, 83, 549-553.

2003

Journal - Research Article

Kammerer, U., Eggert, A. O., Kapp, M., McLellan, A. D., Geijtenbeek, T. B. H., Dietl, J., … Kampgen, E. (2003). Unique appearance of proliferating antigen-presenting cells expressing DC-SIGN (CD209) in the decidua of early human pregnancy. American Journal of Pathology, 162(3), 887-896.

Stoitzner, P., Holzmann, S., McLellan, A. D., Ivarsson, L., Stossel, H., Kapp, M., … Romani, N. (2003). Visualization and characterization of migratory langerhans cells in murine skin and lymph nodes by antibodies against langerin/CD207. Journal of Investigative Dermatology, 120, 266-274.

Leverkus, M., McLellan, A. D., Heldmann, M., Eggert, A. O., Brocker, E. B., Koch, N., & Kampgen, E. (2003). MHC class II-mediated apoptosis in dendritic cells: A role for membrane-associated and mitochondrial signaling pathways. International Immunology, 15(8), 993-1006.

2002

Journal - Research Article

Eggert, A. O., Becker, J. C., Ammon, M., McLellan, A. D., Renner, G., Merkel, A., … Kampgen, E. (2002). Specific peptide-mediated immunity against established melanoma tumors with dendritic cells requires IL-2 and fetal calf serum-free cell culture. European Journal of Immunology, 32(1), 122-127.

McLellan, A. D., Kapp, M., Eggert, A., Linden, C., Bommhardt, U., Brocker, E. B., … Kampgen, E. (2002). Anatomic location and T-cell simulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood, 99(6), 2084-2093.

2001

Journal - Research Article

Schrama, D., Straten, P., Fischer, W. H., McLellan, A. D., Brocker, E. B., Reisfeld, R. A., & Becker, J. C. (2001). Targeting of lymphotoxin-alpha to the tumor elicits an efficient immune response associated with induction of peripheral lymphoid-like tissue. Immunity, 14(2), 111-121.

Kolb-Maurer, A., Pilgrim, S., Kampgen, E., McLellan, A. D., Brocker, E. B., Goebel, W., & Gentschev, I. (2001). Antibodies against listerial protein 60 act as an opsonin for phagocytosis of listeria monocytogenes by human dendritic cells. Infection & Immunity, 69(5), 3100-3109.

2000

Journal - Research Article

Leverkus, M., Walczak, H., McLellan, A., Fries, H. W., Terbeck, G., Brocker, E. B., & Kampgen, E. (2000). Maturation of dendritic cells leads to up-regulation of cellular FLICE-inhibitory protein and concomitant down-regulation of death ligand-mediated apoptosis. Blood, 96(7), 2628-2631.

Kammerer, U., Schoppet, M., McLellan, A. D., Kapp, M., Huppertz, H. I., Kampgen, E., & Dietl, J. (2000). Human decidua contains potent immunostimulatory CD83(+) dendritic cells. American Journal of Pathology, 157(1), 159-169.

McLellan, A., Heldmann, M., Terbeck, G., Weih, F., Linden, C., Brocker, E. B., … Kampgen, E. (2000). MHC class II and CD40 play opposing roles in dendritic cell survival. European Journal of Immunology, 30(9), 2612-2619.

McLellan, A. D., Terbeck, G., Mengling, T., Starling, G. C., Kiener, P. A., Gold, R., … Kampgen, E. (2000). Differential susceptibility to CD95 (Apo-1/Fas) and MHC class II-induced apoptosis during murine dendritic cell development. Cell Death & Differentiation, 7(10), 933-938.

Kato, M., Neil, T. K., Fearnley, D. B., McLellan, A. D., Vuckovic, S., & Hart, D. N. J. (2000). Expression of multilectin receptors and comparative FITC-dextran uptake by human dendritic cells. International Immunology, 12(11), 1511-1519. doi: 10.1093/intimm/12.11.1511

El Sherbini, H., Hock, B., Fearnley, D., McLellan, A., Vuckovic, S., & Hart, D. N. J. (2000). Lectin ligands on human dendritic cells and identification of a peanut agglutinin positive subset in blood. Cellular Immunology, 200, 36-44. doi: 10.1006/cimm.1999.1612

Journal - Research Other

McLellan, A. D., & Kampgen, E. (2000). Functions of myeloid and lymphoid dendritic cells [Review]. Immunology Letters, 72(2), 101-105.

McLellan, A. D., Brocker, E. B., & Kampgen, E. (2000). Dendritic cell activation by danger and antigen specific T-cell signalling [Review]. Experimental Dermatology, 9(5), 313-322.

1999

Journal - Research Article

Hock, B. D., Fearnley, D. B., Boyce, A., McLellan, A. D., Sorg, R. V., Summers, K., & Hart, D. N. J. (1999). Human dendritic cells express a 95 kDa activation/differentiation antigen defined by CMRF-56. Tissue Antigens, 53(4), 320-334. doi: 10.1034/j.1399-0039.1999.530402.x

McLellan, A. D., Heiser, A., & Hart, D. N. J. (1999). Induction of dendritic cell costimulator molecule expression is suppressed by T cells in the absence of antigen specific signalling: Role of cluster formation, CD40 and HLA-Class II for dendritic cell activation. Immunology, 98(2), 171-180. doi: 10.1046/j.1365-2567.1999.00860.x

1998

Journal - Research Article

Sorg, R. V., McLellan, A. D., Hock, B. D., Fearnley, D. B., & Hart, D. N. J. (1998). Human dendritic cells express functional interleukin-7. Immunobiology, 198(5), 514-526. doi: 10.1016/S0171-2985(98)80075-2

McLellan, A. D., Heiser, A., Sorg, R. V., Fearnley, D. B., & Hart, D. N. J. (1998). Dermal dendritic cells associated with T lymphocytes in normal human skin display an activated phenotype. Journal of Investigative Dermatology, 111(5), 841-849. doi: 10.1046/j.1523-1747.1998.00375.x

1997

Journal - Research Article

Fearnley, D. B., McLellan, A. D., Mannering, S. I., Hock, B. D., & Hart, D. N. J. (1997). Isolation of human blood dendritic cells using the CMRF-44 monoclonal antibody: implications for studies on antigen-presenting cell function and immunotherapy. Blood, 89(10), 3708-3726.

Conference Contribution - Published proceedings: Full paper

Hart, D. N., Clark, G., Dekker, J. W., Fearnley, D. B., Kato, M., Hock, B. D., McLellan, A. D., Neil, T. K., Sorg, R. V., Sorg, U. R., Summers, K., & Vuckovic, S. (1997). Dendritic cell surface molecules. A proliferating field. Advances in Experimental and Medical Biology. 417, (pp. 439-442). [Full Paper]

McLellan, A. D., Sorg, R. V., Fearnley, D. B., Hock, B. D., Tiedemann, R. J., Fraser, J. R., & Hart, D. N. J. (1997). T lymphocyte mediated regulation of costimulator molecule expression of human dendritic cells. Advances in Experimental and Medical Biology. 417, (pp. 203-206). [Full Paper]

1996

Journal - Research Article

McLellan, A. D., Sorg, R. V., Williams, L. A., & Hart, D. N. J. (1996). Human dendritic cells activate T lymphocytes via a CD40 : CD40 ligand-dependent pathway. European Journal of Immunology, 26, 1204-1210.

Williams, L. A. M., McLellan, A. D., Summers, K., Sorg, R. V., Fearnley, D. B., & Hart, D. N. J. (1996). Identification of a novel dendritic cell surface antigen defined by carbohydrate specific CD24 antibody cross-reactivity. Immunology, 89, 120-125.