Justin Tirados.
Flow cytometry is used to analyse the physical and chemical characteristics of particles or cells carried in a fluid stream as they pass through at least one laser. Fluorescently-labelled cell components are excited by the laser to emit light at varying wavelengths, the emitted light is measured by detectors.
Flow cytometry is a particularly powerful technique because it allows researchers to rapidly, accurately and simply collect data related to many parameters from a heterogeneous population of cells within a liquid sample.
Read more about:
Initial advice and consultation
Contact for initial advice and consultation:
Email omniflow@otago.ac.nz
Chair of the Flow Cytometry User Group:
Dr Kirsten Ward-Hartstonge
Email kirsten.ward-hartstonge@otago.ac.nz
Services and techniques
- Multi-colour flow cytometry
- Cell sorting
- Analysis workstation
- Training and support resources
- Spectral Cytometry
- Experimental design
- Data analysis
- Sample prep
Equipment
Project discussion with staff is essential prior to registration and training in the use of equipment.
All resources are subject to a minimum booking time of 30 minutes.
For advice on costs please contact michelle.wilson@otago.ac.nz
OMNI equipment use policy (PDF)
Flow cytometric analysers
Sorting (also known as FACS – Fluorescence Assisted Cell Sorting)
- BD FACSAria assisted sorts
FACS analysis
- Analysis Computer and Software (FlowJo, Spice, OMIQ, SpectroFlo)
Technical consultation and services
We can help with all things 'flow' on different levels. We can help design the experiment for you to run or we can come to you and stain, acquire and analyze the data for you depending on your requirements.
Instrument Costs per hour
OMNI reserve the right to alter the costs of the service
Costs below are exclusive of any other consumables or chemicals used, these will be charged separately.
2024 Charges: Health Science Division (HSD) rates only apply to HSD Dunedin campus departments.
| HSD Per Hour | Non-HSD Per Hour | |
|---|---|---|
Flow Cytometry Analysis ASSISTED | $125 | $500 |
Flow Cytometry Analysis UNASSISTED | $65 | $260 |
Flow Cytometry Sorting ASSISTED | $125 | $500 |
Flow Cytometry Data Analysis computer and software | $10 | $45 |
| Technical consultation | $65 | $260 |
Flow Cytometry 3-year projected charges (PDF)
Registration
View our video to learn how to register and book our equipment:
Introductory training
After registering you will have been given access to the University of Otago teams page that contains the following helpful resources;
- Introduction to Flow Cytometry
- Standard operating procedures
- Videos on how to use machines and software
- Flow Cytometry Facility News
- Current and important Flow Cytometry journals
- A forum for discussing Flow Cytometry woes and achievements
- Upcoming talks relating to Flow Cytometry (Basic and Advanced)
Once registered you will be invited to a training session with one of the Flow Cytometry facility staff. University staff and students must have completed their PC2 training in order to attend.
It is recommended you watch the flow beginners' video on Teams before attending training so you have a grasp of what the technique entails.
User Group
The User Group consists of members that represent University of Otago departments that use the Flow Cytometry unit and it advises the OMNI Academic Advisory Group on direction and strategy.
Visit About us for information about how our user groups work and how to make contact.
Flow cytometry events
- Expert Cytometry (ExCyte) offers online webinars on a variety of topics throughout the year
- Cyto University runs online courses throughout the year
Flow cytometry research news
- Early immune response research wins Otago Spotlight Award
- Gene-silencing mechanism may have activation abilities
- Difficult-to-cure malaria target for research
- Humble bacteria shine light on gene editing